Ethanol-type anaerobic digestion enhanced methanogenic performance by stimulating direct interspecies electron transfer and interspecies hydrogen transfer

被引：4
作者：
Zhang, Shuang [1 ]
Ren, Yuanyuan [1 ,2 ]
Zhao, Pan [1 ]
Wang, Xiaona [1 ,2 ]
Wang, Qunhui [1 ,2 ]
Sun, Xiaohong [3 ]
机构：
[1] Univ Sci & Technol Beijing, Sch Energy & Environm Engn, Dept Environm Sci & Engn, Beijing 100083, Peoples R China
[2] Univ Sci & Technol Beijing, Beijing Key Lab Resource oriented Treatment Ind Po, Beijing 100083, Peoples R China
[3] Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2024年 / 410卷
基金：
中国国家自然科学基金;
关键词：
Methane production; E-pili; Ethanol-type fermentation; Syntrophic metabolism; Hydrogenase genes; SYNTROPHIC METABOLISM; FOOD WASTE; FERMENTATION; SLUDGE; PROPIONATE;
D O I：
10.1016/j.biortech.2024.131280
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Ethanol pre-fermentation of food waste effectively alleviates acidification; however, its effects on interspecies electron transfer remain unknown. This study configured the feed according to COD ratios of ethanol: sodium acetate: sodium propionate: sodium butyrate of 5:2:1.5:1.5 (ethanol-type anaerobic digestion) and 0:5:2.5:2.5 (control), and conducted semi-continuous anaerobic digestion (AD) experiments. The results showed that ethanol-type AD increased maximum tolerable organic loading rate (OLR) to 6.0 gCOD/L/d, and increased the methane production by 1.2-14.8 times compared to the control at OLRs of 1.0-5.0 gCOD/L/d. The abundance of the pilA gene, which was associated with direct interspecies electron transfer (DIET), increased by 5.6 times during ethanol-type AD. Hydrogenase genes related to interspecies hydrogen transfer (IHT), including hydA-B, hoxH-Y, hnd, ech, and ehb, were upregulated during ethanol-type AD. Ethanol-type AD improved methanogenic performance and enhanced microbial metabolism by stimulating DIET and IHT.
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页数：11
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