Copy Number Variations of Plasmodium vivax DBP1, EBP/DBP2, and RBP2b in Ethiopians Who Are Duffy Positive and Duffy Negative

被引：0

作者：

Pestana, Kareen ^{[1
]}

Ford, Anthony ^{[2
]}

Rama, Rei

Abagero, Beka ^{[1
]}

Kepple, Daniel ^{[3
]}

Tomida, Junya ^{[3
]}

Popovici, Jean ^{[4
,5
]}

Yewhalaw, Delenasaw ^{[4
,5
]}

Lo, Eugenia ^{[1
]}

机构：

[1] Drexel Univ, Coll Med, Dept Microbiol & Immunol, 2900 W Queen Lane,G50, Philadelphia, PA 19129 USA

[2] Univ North Carolina Charlotte, Dept Bioinformat & Genom, Charlotte, NC USA

[3] Univ North Carolina Charlotte, Biol Sci, Charlotte, NC USA

[4] Inst Pasteur, Inst Pasteur Cambodge, Malaria Mol Epidemiol Unit, Malaria Translat Res Unit, Phnom Penh, Cambodia

[5] Inst Pasteur, Infect Dis Epidemiol & Analyt, Paris, France

来源：

JOURNAL OF INFECTIOUS DISEASES | 2024年 / 230卷 / 04期

基金：

美国国家卫生研究院;

关键词：

CNV; Plasmodium vivax; PvDBP1; PvEBP/DBP2; PvRBP2b; MEFLOQUINE RESISTANCE; AMPLIFICATION; GENE; INDIVIDUALS; FALCIPARUM; INVASION; MALARIA; BLOOD;

D O I：

10.1093/infdis/jiae388

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recent evidence challenges the belief that individuals who are Duffy-negative are resistant to Plasmodium vivax due to lacking the Duffy antigen receptor for chemokines. Erythrocyte-binding protein (EBP/DBP2) has shown moderate binding to Duffy-negative erythrocytes in vitro. Reticulocyte-binding protein 2b (RBP2b) interactions with transferrin receptor 1 suggest involvement in Duffy-negative infections. Gene copy number variations in PvDBP1, PvEBP/DBP2, and PvRBP2b were investigated in Duffy-positive and Duffy-negative P vivax infections from Ethiopia. Among Duffy-positive samples, 34% displayed PvDBP1 duplications (Cambodian type). In Duffy-negative infections, 30% showed duplications, mostly Cambodian type. For PvEBP/DBP2 and PvRBP2b, Duffy-positive samples exhibited higher duplication rates (1-8 copies for PvEBP/DBP2, 46%; 1-5 copies for PvRBP2b, 43%) as compared with Duffy-negative samples (20.8% and 26%, respectively). The range of copy number variations was lower in Duffy-negative infections. Demographic and clinical factors associated with gene multiplications in both Duffy types were explored, enhancing understanding of P vivax evolution in Africans who are Duffy negative. This study investigates gene copy number variations in Plasmodium vivax DBP1, EBP/DBP2, and RBP2b among Ethiopians who were Duffy positive and Duffy negative, revealing significant differences in gene duplications and providing insights into the parasite's evolution and infection mechanisms.

引用

页码：1004 / 1012

页数：9