Long-term stability of frozen platelet-rich plasma under-80 °C storage condition

被引:0
作者
Liu, Wanbing [1 ]
Liu, Yan [2 ]
Li, Tao [3 ]
Liu, Lei [1 ]
Du, Mei [4 ]
Du, Jinbing [1 ]
Qi, Yong [1 ]
Xiang, Guangda [3 ]
机构
[1] Gen Hosp Cent Theater Command, Dept Transfus, Wuluo Rd 627, Wuhan 430070, Hubei, Peoples R China
[2] Maternal & Child Hlth Hosp Hubei Prov, Dept Clin Lab, Wuluo Rd 745, Wuhan 430070, Hubei, Peoples R China
[3] Gen Hosp Cent Theater Command, Dept Endocrinol, Wuluo Rd 627, Wuhan 430070, Hubei, Peoples R China
[4] Maternal & Child Hlth Hosp Hubei Prov, Dept Gynecol 2, Wuluo Rd 745, Wuhan 430070, Hubei, Peoples R China
基金
中国博士后科学基金;
关键词
Platelet rich plasma; Frozen storage; Platelet-derived growth factor (PDGF); Vascular endothelial growth factor (VEGF); Epidermal growth factor (EGF); Basic fibroblast growth factor (bFGF); EARLY OSTEOARTHRITIS; INJECTIONS;
D O I
10.1016/j.reth.2024.09.006
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Platelet rich plasma (PRP) is increasingly used in various fields of medicine, aiming to regeneration and repair damaged tissues, cells and organs. High concentration of bioactive molecules including growth factors, cytokines and chemokines are the rationale of using PRP. The aim of this study is to analyze the effect of frozen on the levels of growth factors. In our study, PRP samples were isolated from 50 healthy volunteers using the Trima Accel blood cell separator. The concentration of growth factors such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF-1) and platelet factor 4 (PF-4) were assessed in fresh PRP and frozen PRP stored at -80 degrees C for one to twelve months. The study found that count of platelet in all fresh and frozen PRP samples was significantly increased compared to whole blood baseline. There was no significant difference in the concentrations of PDGF-BB, bFGF, VEGF, and PF-4 between fresh and frozen samples. The concentrations of EGF and IGF in Frozen-PRP group were significantly higher than those in Fresh-PRP group. And the storage condition of -80 degrees C is suitable for PRP, which will not lead to a decrease in growth factors concentration for at least 6 months. (c) 2024 The Author(s). Published by Elsevier BV on behalf of The Japanese Society for Regenerative Medicine. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/).
引用
收藏
页码:826 / 830
页数:5
相关论文
共 43 条
[41]   Additional Intraoperative Autologous-Derived Platelet-Rich Stroma to Transanal Flap Repair for the Treatment of Cryptoglandular Transsphincteric Fistulas in a Tertiary Referral Center: Long-Term Outcomes of a Prospective Pilot Study [J].
Bak, Michiel T. J. ;
Witjes, Caroline D. M. ;
Dwarkasing, Roy S. ;
Arkenbosch, Jeanine H. C. ;
Schouten, W. Rudolph ;
van Veen, Jochem C. ;
van Dongen, Joris A. ;
Fuhler, Gwenny M. ;
van der Woude, C. Janneke ;
de Vries, Annemarie C. ;
van Ruler, Oddeke .
BIOENGINEERING-BASEL, 2025, 12 (02)
[42]   In vitro effect of platelet-rich plasma (PRP) on morpho-functional characteristics, oxidative stress, apoptosis and CatSper-1 ion channel in short-term stored (5 °C) ram semen [J].
Turk, Gaffari ;
Gungor, Ibrahim brahim Halil ;
Arkali, Gozde ;
Cihangiroglu, Aslihan Cakir ;
Badilli, Nida ;
Acisu, Tutku Can ;
Cinkara, Serap Dayan ;
Yuksel, Suheda ;
Kaya, Seyma Ozer ;
Sonmez, Mustafa ;
Gur, Seyfettin ;
Yuce, Abdurrauf .
SMALL RUMINANT RESEARCH, 2025, 244
[43]   Intraoperative Autologous Platelet-Rich Stroma Injection as Add-On to Fistula Curettage and Closure of the Internal Orifice Demonstrates a Favorable Outcome in Long-Term in Patients Suffering from Therapy-Refractory Perianal Fistulizing Crohn's Disease [J].
Bak, Michiel T. J. ;
de Vries, Annemarie C. ;
Witjes, Caroline D. M. ;
Arkenbosch, Jeanine H. C. ;
Dwarkasing, Roy S. ;
van Dongen, Joris A. ;
Fuhler, Gwenny M. ;
Schouten, Willem Rudolph ;
van der Woude, Christien Janneke ;
van Ruler, Oddeke .
INFLAMMATORY BOWEL DISEASES, 2025,