Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3

被引：1

作者：

Tommeurd, Wantanee ^{[1
]}

Thueng-in, Kanyarat ^{[2
]}

Theerawatanasirikul, Sirin ^{[1
,3
]}

Tuyapala, Nongnaput ^{[4
]}

Poonsuk, Sukontip ^{[5
]}

Petcharat, Nantawan ^{[6
]}

Thangthamniyom, Nattarat ^{[7
]}

Lekcharoensuk, Porntippa ^{[1
,6
]}

机构：

[1] Kasetsart Univ, Grad Sch, Interdisciplinary Grad Program Genet Engn, Bangkok 10900, Thailand

[2] Suranaree Univ Technol, Inst Med, Sch Pathol, Translat Med Program, Nakhon Ratchasima 30000, Thailand

[3] Kasetsart Univ, Fac Vet Med, Dept Anat, Bangkok 10900, Thailand

[4] Natl Sci & Technol Dev Agcy NSTDA, Natl Ctr Genet Engn & Biotechnol BIOTEC, Prot Ligand Engn & Mol Biol Res Team, Khlong Nueng 12120, Pathum Thani, Thailand

[5] Kasetsart Univ, Fac Vet Med, Dept Pathol, Bangkok 10900, Thailand

[6] Kasetsart Univ, Fac Vet Med, Dept Microbiol & Immunol, Bangkok 10900, Thailand

[7] CPF Thailand Publ Co Ltd, Anim Hlth & Diagnost Ctr, Res & Dev Dept, Bangkok 10530, Thailand

来源：

ANTIBODIES | 2024年 / 13卷 / 03期

关键词：

foot-and-mouth disease virus; monoclonal antibody; hybridoma; phage display; CONFORMATIONAL EPITOPE; NEUTRALIZING EPITOPES; STRUCTURAL PROTEINS; VISUALIZATION; BINDING; VP2;

D O I：

10.3390/antib13030067

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Foot-and-mouth disease (FMD) is a highly infectious disease of cloven-hoofed animals with a significant economic impact. Early diagnosis and effective prevention and control could reduce the spread of the disease which could possibly minimize economic losses. Epitope characterization based on monoclonal antibodies provide essential information for developing diagnostic assays and vaccine designs. In this study, monoclonal antibodies raised against FMD virus (FMDV) were produced. Sixty-six monoclonal antibodies demonstrated strong reactivity and specificity to FMDV. The purified monoclonal antibodies were further used for bio-panning to select phage expressing specific epitopes from phage-displayed 12 mer-peptide library. The phage peptide sequences were analyzed using multiple sequence alignment and evaluated by peptide ELISA. Two hybridoma clones secreted monoclonal antibodies recognizing linear epitopes on VP2 of FMDV serotype O. The non-neutralizing monoclonal antibody 6F4.D11.B6 recognized the residues 67-78 on antigenic site 2 resinding in VP2, while the neutralizing monoclonal antibody 8D6.B9.C3 recognized a novel linear epitope encompassing residues 115-126 on VP2. This information and the FMDV-specific monoclonal antibodies provide valuable sources for further study and application in diagnosis, therapeutics and vaccine designs to strengthen the disease prevention and control measures.

引用

页数：16