Wound healing effects of biogenic gold nanoparticles synthesized using red wine extracts

被引：0

作者：

Mgijima, Tswellang ^{[1
]}

Sibuyi, Nicole R. S. ^{[2
,3
]}

Fadaka, Adewale O. ^{[2
]}

Meyer, Samantha ^{[4
]}

Madiehe, Abram M. ^{[2
]}

Meyer, Mervin ^{[2
]}

Onani, Martin O. ^{[1
]}

机构：

[1] Univ Western Cape, Dept Chem Sci, Organometall & Nanomat, Bellville, South Africa

[2] Univ Western Cape, Mintek Nanotechnol Innovat Ctr NIC, Dept Sci & Innovat DSI, Dept Biotechnol,Biolabels Res Node, Bellville, South Africa

[3] Mintek, Adv Mat Div, Hlth Platform, Randburg, South Africa

[4] Cape Peninsula Univ Technol, Dept Biomed Sci, Phytotherapy Res Grp, Bellville, South Africa

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2024年 / 52卷 / 01期

关键词：

gold nanoparticles; green synthesis; wound healing; Cabernet sauvignon red wine; pinot noir red wine; pinotagered wine; IN-VITRO; SILVER; ANTIBACTERIAL; SKIN; BIOSYNTHESIS; SPECTROSCOPY; SIZE;

D O I：

10.1080/21691401.2024.2383583

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Gold nanoparticles (AuNPs) were synthesized using three red wine extracts (RW-Es); by varying temperature, pH, concentrations of RW-Es and gold salt. The RW-AuNPs were characterized by UV-vis, transmission electron microscopy (TEM), dynamic light scattering (DLS), and the Fourier Transform Infra-red Spectroscopy (FT-IR). Their stability was evaluated in water, foetal bovine serum (FBS), phosphate-buffered saline (PBS), and Dulbecco's Modified Eagle Medium (DMEM) by UV-Vis. The effect of the RW-Es and RW-AuNPs on KMST-6 cell cell viability was evaluated by MTT assay; and their wound healing effects were monitored by scratch assay. RW-AuNPs synthesis was observed by colour change, and confirmed by UV-Vis spectrum, with an absorption peak around 550 nm. The hydrodynamic sizes of the RW-AuNPs ranged between 10 and 100 nm. Polyphenols, carboxylic acids, and amino acids are some of functional groups in the RW-Es that were involved in the reduction of RW-AuNPs. The RW-AuNPs were stable in test solutions and showed no cytotoxicity to the KMST-6 cells up to 72 h. AuNPs synthesized from Pinotage and Cabernet Sauvignon enhanced proliferation of KMST-6 cells and showed potential as wound healing agents. Further studies are required to investigate the molecular mechanisms involved in the potential wound-healing effect of the RW-AuNPs.

引用

页码：399 / 410

页数：12