Phytosterols in mountain cultivated ginseng, as master healthy ageing dietary supplement, activates steroid signaling in ageing Drosophila melanogaster

被引：0

作者：

Liu, Jialiang ^{[1
]}

Ma, Rui ^{[1
]}

Fu, Baoyu ^{[1
]}

Yang, Pengdi ^{[1
]}

Zhang, Lili ^{[1
]}

Zhang, Chunyang ^{[2
]}

Chen, Ying ^{[1
]}

Sun, Liwei ^{[1
,3
]}

机构：

[1] Changchun Univ Chinese Med, Affiliated Hosp, Changchun 130021, Jilin Province, Peoples R China

[2] Jilin Prov Sericultural Sci Res Inst, Jilin 132012, Peoples R China

[3] Changchun Univ Chinese Med, Key Lab Act Subst & Biol Mech Ginseng Efficacy, Minist Educ, Changchun 130117, Jilin Province, Peoples R China

来源：

EXPERIMENTAL GERONTOLOGY | 2024年 / 195卷

基金：

中国国家自然科学基金;

关键词：

Phytosterols; Mountain cultivated ginseng; Healthy ageing; Steroid signaling; Stigmasterol; PLANT STEROLS;

D O I：

10.1016/j.exger.2024.112554

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Mountain cultivated ginseng (MCG) is planted in mountain forests to simulate traditional wild ginseng; therefore, it has a greater pharmacological effect than cultivated ginseng (CG) in the garden; however, insufficient evidence confirms this theory. In light of the health-promoting and life-extending properties of ginseng, we analyzed the efficacy of MCG and CG. Initial observations revealed that the phytosterols content of MCG was higher than that of CG, with a positive correlation to the duration of growth. The distinction between phytosterols in MCG and in CG is predominately determined by the stigmasterol content using High-Performance Liquid Chromatography (HPLC). The lifespan of Drosophila melanogaster (fruit flies) that aged naturally was prolonged by phytosterols in MCG and CG and stigmasterols. Further, they prolonged healthy ageing as measured by progeny numbers, length of sleep, climbing distance, and survival following oxidative damage. The findings of behavioral observations revealed that phytosterols in MCG were more efficacious than in CG in promoting health maintenance and life extension; moreover, stigmasterol indicated that these effects were dose-dependent. Stigmasterols, phytosterols in MCG and CG have restored age-associated decreases in steroid hormone levels. Notably, molecular docking was predicted to promote stigmasterol's binding to the steroid hormone receptor ECR due to its similarity to steroid hormones. In addition, stigmasterols triggered the steroid hormone signaling pathway by increasing the activity of key genes Eip75B and Br in 20E signaling and Jhamt, HmGR, Met, and Kr-h1 in JH signaling. Phytosterols, as a natural product, regulated health and longevity as a dietary supplement similar to that of steroids, which supported the social requirements of healthy ageing.

引用

页数：9