Systems genetic analysis of lignin biosynthesis in Populus tremula

被引：0

作者：

Luomaranta, Mikko ^{[1
]}

Grones, Carolin ^{[1
]}

Choudhary, Shruti ^{[2
]}

Milhinhos, Ana ^{[1
]}

Kalman, Teitur Ahlgren ^{[1
]}

Nilsson, Ove ^{[2
]}

Robinson, Kathryn M. ^{[1
]}

Street, Nathaniel R. ^{[1
,3
]}

Tuominen, Hannele ^{[2
]}

机构：

[1] Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, S-90187 Umea, Sweden

[2] Swedish Univ Agr Sci, Umea Plant Sci Ctr, Dept Forest Genet & Plant Physiol, S-90183 Umea, Sweden

[3] Umea Univ, SciLifeLab, S-90187 Umea, Sweden

来源：

NEW PHYTOLOGIST | 2024年 / 243卷 / 06期

关键词：

aspen; eQTL; GWAS; HD-Zip III; lignin biosynthesis; Populus; wood formation; GENOME-WIDE ASSOCIATION; BIOMASS RECALCITRANCE; EXPRESSION; WOOD; VARIANTS; REVEAL; LIGNIFICATION; PROMOTER; ACCURACY; MUTATION;

D O I：

10.1111/nph.19993

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The genetic control underlying natural variation in lignin content and composition in trees is not fully understood. We performed a systems genetic analysis to uncover the genetic regulation of lignin biosynthesis in a natural 'SwAsp' population of aspen (Populus tremula) trees. We analyzed gene expression by RNA sequencing (RNA-seq) in differentiating xylem tissues, and lignin content and composition using Pyrolysis-GC-MS in mature wood of 268 trees from 99 genotypes. Abundant variation was observed for lignin content and composition, and genome-wide association study identified proteins in the pentose phosphate pathway and arabinogalactan protein glycosylation among the top-ranked genes that are associated with these traits. Variation in gene expression and the associated genetic polymorphism was revealed through the identification of 312 705 local and 292 003 distant expression quantitative trait loci (eQTL). A co-expression network analysis suggested modularization of lignin biosynthesis and novel functions for the lignin-biosynthetic CINNAMYL ALCOHOL DEHYDROGENASE 2 and CAFFEOYL-CoA O-METHYLTRANSFERASE 3. PHENYLALANINE AMMONIA LYASE 3 was co-expressed with HOMEOBOX PROTEIN 5 (HB5), and the role of HB5 in stimulating lignification was demonstrated in transgenic trees. The systems genetic approach allowed linking natural variation in lignin biosynthesis to trees<acute accent> responses to external cues such as mechanical stimulus and nutrient availability.

引用

页码：2157 / 2174

页数：18

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