Fabrication of nanomolecular platform based immunosensor for non-invasive electrochemical detection of oral cancer: An in vitro study

被引：1

作者：

Choudhary, Meenakshi ^{[1
,2
,3
]}

Kumar, Vinay ^{[4
]}

Parambath, Ashik Babu ^{[5
]}

Caplash, Shruti ^{[3
]}

Yadav, Birendra Kumar ^{[6
,7
]}

Kaur, Satbir ^{[3
]}

Shah, Sapna Ratan ^{[5
]}

Arora, Kavita ^{[1
,5
]}

机构：

[1] Jawaharlal Nehru Univ JNU, Adv Instrumentat Res Facil, New Delhi 110067, India

[2] Indian Inst Technol Delhi IITD, Ctr Biomed Engn, New Delhi 110016, India

[3] Punjabi Univ Patiala, Dept Human Genet, Patiala 147002, Punjab, India

[4] Penn State Univ, Hershey Med Ctr, 500 Univ Dr, Hershey, PA 17033 USA

[5] Jawaharlal Nehru Univ JNU, Sch Computat & Integrat Sci, New Delhi 110067, India

[6] Bio Bank India Fdn BBIF, Virtual Biobank, New Delhi, India

[7] Inst Liver & Billiary Sci ILBS, Natl Liver Dis Biobank, New Delhi, India

来源：

TALANTA OPEN | 2024年 / 10卷

关键词：

Oral cancer; Immunosensor; S100; Silver nanoparticles; Chitosan; Metal-organic frameworks; Point-of-care diagnosis; DIAGNOSIS;

D O I：

10.1016/j.talo.2024.100352

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Silver nanoparticles (AgNPs)-Chitosan (Chi) functionalized cysteine (cys)-SAM (self-assembled monolayer) based electrochemical, label free, ultrasensitive, non-invasive immunosensing nanomolecular platform has been fabricated for the detection of novel OC biomarker S-100. This has been achieved through bio-functionalization of AgNPs-Chi/cys/mini gold slides by covalent immobilization of anti-S100 antibodies. Cyclic voltammetric (CV) studies establish successful fabrication of anti-S100/AgNPs-Chi/Au nanomolecular platform. Differential pulse voltammetric (DPV) measurements reveal that anti-S100/Chi-AgNPs/Au is able to sense 50 fg mL(-1 )- 500 ng mL(-1) of S100 in buffer and in spiked saliva samples within 120 s interaction time having improved favorable shelf life and specificity. The fabricated platform is very selective and able to detect S100 in OC patient/s saliva samples warrants realization of simple non-invasive electrochemical immunosensor for in situ or on-site applications (especially for remote areas) for early diagnosis of OC while offering bio-compatibility at low cost.

引用

页数：10

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