Applications of Single-Cell Omics Technologies for Induced Pluripotent Stem Cell-Based Cardiovascular Research

被引：0

作者：

Kim, Hyunjoon ^{[1
,2
]}

Choi, Sohee ^{[2
,3
]}

Heo, HyoJung ^{[1
,2
]}

Cho, Su Han ^{[2
,4
]}

Lee, Yuna ^{[2
,5
]}

Kim, Dohyup ^{[2
,6
]}

Jung, Kyung Oh ^{[2
,7
]}

Rhee, Siyeon ^{[2
,8
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA USA

[2] K BioX, Palo Alto, CA USA

[3] Sookmyung Womens Univ, Dept Biol Sci, Seoul, South Korea

[4] Kyung Hee Univ, Dept Biol, Seoul, South Korea

[5] Konkuk Univ, Dept Syst Biotechnol, Seoul, South Korea

[6] Cincinnati Childrens Hosp Med Ctr, Asthma Res Div, Cincinnati, OH USA

[7] Chung Ang Univ, Coll Med, Dept Anat, Seoul, South Korea

[8] Stanford Univ, Sch Med, Stanford Cardiovasc Inst, 240 Pasteur Dr, Palo Alto, CA 94304 USA

来源：

INTERNATIONAL JOURNAL OF STEM CELLS | 2024年

基金：

美国国家卫生研究院;

关键词：

Transcriptome analysis; Induced pluripotent stem cells; Organoids; Cardiovascular diseases; REVEALS; HEART; DIFFERENTIATION; PROTEINS;

D O I：

10.15283/ijsc23183

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Single-cell omics technologies have transformed our investigation of genomic, transcriptomic, and proteomic landscapes at the individual cell level. In particular, the application of single-cell RNA sequencing has unveiled the complex transcriptional variations inherent in cardiac cells, offering valuable perspectives into their dynamics. This review focuses on the integration of single-cell omics with induced pluripotent stem cells (iPSCs) in the context of cardiovascular research, offering a unique avenue to deepen our understanding of cardiac biology. By synthesizing insights from various single-cell technologies, we aim to elucidate the molecular intricacies of heart health and diseases. Beyond current methodologies, we explore the potential of emerging paradigms such as single-cell/spatial omics, delving into their capacity to reveal the spatial organization of cellular components within cardiac tissues. Furthermore, we anticipate their transformative role in shaping the future of cardiovascular research. This review aims to contribute to the advancement of knowledge in the field, offering a comprehensive perspective on the synergistic potential of transcriptomic analyses, iPSC applications, and the evolving frontier of spatial omics.

引用

页码：37 / 48

页数：11