Biological and genomic characterization of a polyvalent phage PSH-1 against multidrug-resistant Salmonella Enteritidis

被引:0
|
作者
Li, Shuai-Hua [1 ]
Wang, Rui-Yun [1 ]
Zhang, Jun-Kai [1 ]
Yi, Kai-Fang [1 ]
Liu, Jian-Hua [1 ]
Wu, Hua [1 ]
Yuan, Li [1 ]
Zhai, Ya-Jun [1 ]
Hu, Gong-Zheng [1 ]
机构
[1] Henan Agr Univ, Coll Anim Sci & Vet Med, Dept Pharmacol & Toxicol, Zhengzhou, Peoples R China
来源
BMC MICROBIOLOGY | 2024年 / 24卷 / 01期
关键词
Multidrug-resistant Salmonella Enteritidis; Polyvalent phage; Biological characteristics; Genomic analysis; Food bacteriostatic test; HOST-RANGE; BACTERIOPHAGE;
D O I
10.1186/s12866-024-03489-w
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background Bacteriophage has been renewed attention as a new antibacterial agent due to the limitations of antibiotic treatment. Bacteriophages are generally thought to be highly host specific and even strain specific, but a small number of polyvalent bacteriophages have been found to infect bacteria of different genera. Results In this study, a virulent lytic bacteriophage (named Salmonella phage PSH-1) of Salmonella Enteritidis was isolated from the sewage samples of a large-scale pig farm, PSH-1 demonstrated lytic activity against four multidrug-resistant Salmonella Enteritidis isolates and Escherichia coli, and then its biological characteristics, genome and bacteriostatic ability were investigated. The results showed that the initial titer of PSH-1 was 1.15 x 1010 PFU/mL and the optimal multiplicity of infection (MOI) was 0.01, PSH-1 has stable activity in the range of pH 3.0-11.0. One-step growth curve showed that its latent period was 20 min, burst time was 80 min, and the burst was 495 particles. The whole-genome sequencing results revealed phage PSH-1 had a linear dsDNA with 48,466 bp length. The G/C content was 45.33%. Non-coding RNA genes and virulence factors were not found. Eighty- five open reading frames (ORFs) were identified after online annotation. By tests, the use of phage could succeed in controlling the artificial Salmonella contamination in milk at a range of temperatures. Conclusions This study reports a novel Salmonella Enteritidis phage PSH-1, which has a robust lytic ability, no virulence factors, and good stability. The characterization and genomic analysis of PSH-1 will develop our understanding of phage biology and diversity and provide a potential arsenal for controlling of salmonellosis.
引用
收藏
页数:12
相关论文
共 50 条
  • [41] Characterization and Genomic Study of Phage vB_ EcoS-B2 Infecting Multidrug-Resistant Escherichia coli
    Xu, Yue
    Yu, Xinyan
    Gu, Yu
    Huang, Xu
    Liu, Genyan
    Liu, Xiaoqiu
    FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [42] Synergistic influence of probiotic and florfenicol on embryonic viability, performance, and multidrug-resistant Salmonella Enteritidis in broiler chickens
    Nabil, Nehal M.
    Tawakol, Maram M.
    Samir, Abdelhafez
    Hassan, Heba M.
    Yonis, Ahlam E.
    Reda, Reem M.
    Elsayed, Mona M.
    SCIENTIFIC REPORTS, 2023, 13 (01)
  • [43] Synergistic influence of probiotic and florfenicol on embryonic viability, performance, and multidrug-resistant Salmonella Enteritidis in broiler chickens
    Nehal M. Nabil
    Maram M. Tawakol
    Abdelhafez Samir
    Heba M. Hassan
    Ahlam E. Yonis
    Reem M. Reda
    Mona M. Elsayed
    Scientific Reports, 13
  • [44] Genomic Characterization of Multidrug-Resistant Acinetobacter baumannii in Pneumonia Patients in Kazakhstan
    Strochkov, Vitaliy
    Beloussov, Vyacheslav
    Orkara, Shynggys
    Lavrinenko, Alyona
    Solomadin, Maxim
    Yegorov, Sergey
    Sandybayev, Nurlan
    DIAGNOSTICS, 2025, 15 (06)
  • [45] Complete Genome Sequence of Salmonella Phage vB_SenA_SM5, Active against Multidrug-Resistant Salmonella enterica Serovar Typhi Isolates
    Chaudhary, Naveen
    Singh, Dharminder
    Maurya, Ravi Kumar
    Mohan, Balvinder
    Taneja, Neelam
    MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2022, 11 (07):
  • [46] Phage-Derived Depolymerase as an Antibiotic Adjuvant Against Multidrug-Resistant Acinetobacter baumannii
    Chen, Xi
    Liu, Miao
    Zhang, Pengfei
    Xu, Miao
    Yuan, Weihao
    Bian, Liming
    Liu, Yannan
    Xia, Jiang
    Leung, Sharon S. Y.
    FRONTIERS IN MICROBIOLOGY, 2022, 13
  • [47] Phage-derived lysins as therapeutic agents against multidrug-resistant Enterococcus faecalis
    Gu, Jingmin
    Xi, Hengyu
    Cheng, Mengjun
    Han, Wenyu
    FUTURE MICROBIOLOGY, 2018, 13 (03) : 275 - 278
  • [48] Characterization of the emerging multidrug-resistant Salmonella enterica serovar Indiana strains in China
    Gong, Jiansen
    Zeng, Ximin
    Zhang, Ping
    Zhang, Di
    Wang, Chengming
    Lin, Jun
    EMERGING MICROBES & INFECTIONS, 2019, 8 (01): : 29 - 39
  • [49] Characterization and biological investigation of silver nanoparticles biosynthesized from Galaxaura rugosa against multidrug-resistant bacteria
    Alzahrani, Raghad R.
    Alkhulaifi, Manal M.
    Alenazi, Nouf M.
    Almusayeib, Nawal M.
    Amina, Musarat
    Awad, Manal A.
    Elmubarak, Aarif H.
    Aldosari, Noura S.
    JOURNAL OF TAIBAH UNIVERSITY FOR SCIENCE, 2020, 14 (01): : 1651 - 1659
  • [50] Characterization and therapeutic potential of phage vB_Eco_ZCEC08 against multidrug-resistant uropathogenic Escherichia coli
    Assmaa H. Hussein
    Salsabil Makky
    Raghda Hager
    Ian F. Connerton
    Ayman El-Shibiny
    BMC Microbiology, 25 (1)