Parallel reaction monitoring targeted mass spectrometry as a fast and sensitive alternative to antibody-based protein detection

被引:0
|
作者
Bezstarosti, Karel [1 ]
van der Wal, Lennart [1 ]
Doff, Wouter A. S. [1 ]
Demmers, Jeroen A. A. [1 ]
机构
[1] Erasmus Univ, Med Ctr, Prote Ctr, Rotterdam, Netherlands
来源
关键词
parallel reaction monitoring (PRM); targeted mass spectrometry; immunoblotting; limit of detection; GAPDH; ELECTROPHORETIC TRANSFER; POLYACRYLAMIDE-GELS; PROTEOMICS; MRM; NITROCELLULOSE; QUANTIFICATION; ACCURACY;
D O I
10.3389/frans.2024.1397810
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The reliable, accurate and quantitative targeted detection of proteins is a key technology in molecular and cell biology and molecular diagnostics. The current golden standard for targeted protein detection in complex mixtures such as complete cell lysates or body fluids is immunoblotting, a technology that was developed in the late 1970s and has not undergone major changes since. Although widespread, this methodology suffers from several disadvantages, such as the inability to detect low-abundant proteins or specific posttranslational modifications, the requirement for highly specific antibodies, the lack of quantitative power and the often-tedious practical procedures. Mass spectrometry (MS) based targeted protein detection is an alternative technology that could circumvent these caveats. Here, we compare immunoblotting with targeted protein mass spectrometry using a parallel reaction monitoring (PRM) regime on the Orbitrap mass spectrometer. We show that PRM based MS has superior sensitivity and quantitative accuracy over immunoblotting. The limit of detection for proteolytic peptides of a purified target protein was found to be in the mid- to low-attomole range and approximately one order of magnitude higher when embedded in a complex biological matrix. The incorporation of synthetic heavy isotope labeled (AQUA) peptides as internal calibrants into the PRM workflow allows for even higher accuracy for both the relative and absolute quantitation of tryptic target peptides. In conclusion, PRM is a versatile and sensitive technology, which can overcome the shortcomings of immunoblotting. We argue that PRM based MS could become the method of choice for the targeted detection of proteins in complex cellular matrices or body fluids and may eventually replace standard methods such as Western blotting and ELISA in biomedical research and in the clinic.
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页数:11
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