Role of superhydrophobicity in the biological activity of fibronectin at the cell-material interface

被引:59
作者
Ballester-Beltran, Jose [1 ]
Rico, Patricia [1 ,2 ]
Moratal, David [1 ]
Song, Wenlong [3 ,4 ]
Mano, Joao F. [3 ,4 ]
Salmeron-Sanchez, Manuel [1 ,2 ,5 ]
机构
[1] Univ Politecn Valencia, Ctr Biomat & Tissue Engn, E-46071 Valencia, Spain
[2] CIBER BBN, Valencia, Spain
[3] Univ Minho, Res Grp Biomat Biodegradables & Biomimet 3Bs, Headquarters European Inst Excellence Tissue Engn, Dept Polymer Engn, Guimaraes, Portugal
[4] PT Associated Lab, IBB, Guimaraes, Portugal
[5] Ctr Invest Principe Felipe, Regenerat Med Unit, Valencia, Spain
关键词
FOCAL ADHESION KINASE; SUBSTRATUM-BOUND FIBRONECTIN; SELF-ASSEMBLED MONOLAYERS; SURFACE-ENERGY; OSTEOBLAST ADHESION; FIBROBLAST ADHESION; PROTEIN ADSORPTION; INTEGRIN BINDING; MATRIX; ORGANIZATION;
D O I
10.1039/c1sm06102j
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Protein adsorption and cellular behavior depend strongly on the wettability of substrates. Such studies are scarce for surfaces exhibiting extreme values of contact angles. Fibronectin (FN) adsorption and adhesion of MC3T3-E1 cells were investigated on superhydrophobic polystyrene (SH-PS) surfaces and compared with the corresponding smooth polystyrene (PS) substrate and the control glass. The FN surface density was lower on the SH-PS than on PS, and the adsorbed protein showed altered conformation of cell adhesion domains, as obtained by ELISA with monoclonal antibodies. Cell adhesion occurred on the SH-PS without the formation of mature focal adhesions, as assessed by immunofluorescence for vinculin, talin and paxillin. Correspondingly, the development of the actin cytoskeleton was delayed and without the presence of defined F-actin fibers. FAK phosphorylation was reduced on SH-PS, as compared with PS and the control glass. Also, cell contractility was diminished on the SH-PS as revealed by phosphorylation of myosin light chain (pMLC). Likewise, FN reorganization and secretion were impaired on the superhydrophobic surfaces. Cell proliferation was significantly lower in SH-PS as compared with PS up to 21 days of culture.
引用
收藏
页码:10803 / 10811
页数:9
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