The effect of sewage source on HF183 risk-based threshold estimation for recreational water quality management

Host-associated fecal indicator measurements can be coupled with quantitative microbial risk assessment to develop risk-based thresholds for recreational use of potential sewage-contaminated waters. These assessments require information on the relative concentrations of indicators and pathogens in discharged sewage, typically based on data collected from wastewater treatment plant influent samples. However, most untreated sewage releases occur from within the collection system itself (i.e. compromised sewer laterals, compromised gravity and force mains, sanitary sewer overflows), where these relationships may differ. This study therefore analyzed the concentrations of a selected reference pathogen (norovirus) and fecal indicator (HF183) in sewage samples from upper and lower segments of gravity sewage collection systems, wastewater pumpstations, and the influent and effluent of treatment plants, to characterize variability in their relative concentrations. Norovirus detection rates were lower and more variable in upper collection system samples due to the smaller population represented; whereas, HF183 was routinely detected at all sites with higher concentrations in the collection system compared to treatment plant influent, resulting in variable comparative relationships across sample locations (types). Mean HF183:NoV ratios ranged from 1.0 x 105 for sewer lateral samples to 7 x 10 degrees for force main samples. Results were used to develop risk-based thresholds for HF183 based on estimated recreational exposure to norovirus following a release from each potential sewage source, with higher thresholds for treatment facility influent compared to forced mains, or effluent. Consequently, this approach can allow for the rapid application of potential risk-based thresholds for recreational water quality applications based on different types of sewage discharge events.