N6-methyladenosine-mediated overexpression of TREM-1 is associated with periodontal disease

被引:0
|
作者
Burra Anand, Deepika [1 ]
Ramamurthy, Jaiganesh [1 ]
Kannan, Balachander [2 ]
Jayaseelan, Vijayashree Priyadharsini [3 ]
Arumugam, Paramasivam [2 ]
机构
[1] Saveetha Univ, Saveetha Inst Med & Tech Sci SIMATS, Saveetha Dent Coll & Hosp, Dept Periodont, Chennai, India
[2] Saveetha Univ, Saveetha Dent Coll & Hosp, Saveetha Inst Med & Tech Sci SIMATS, Ctr Cellular & Mol Res,Mol Biol Lab, Chennai 600077, India
[3] Saveetha Univ, Saveetha Dent Coll & Hosp, Saveetha Inst Med & Tech Sci SIMATS, Ctr Cellular & Mol Res,Clin Genet Lab, Chennai, India
关键词
Periodontitis; Healthcare; Genetics; TREM1; m6A; METTL3; PERI-IMPLANT DISEASES; 2017 WORLD WORKSHOP; MYELOID CELLS; CONSENSUS REPORT; ACTIVATION; CLASSIFICATION; PATHWAYS;
D O I
10.1007/s10266-024-01009-w
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Periodontitis, a prevalent inflammatory disease, involves the destruction of tooth-supporting tissues. N6-methyladenosine (m6A) is a type of post-transcriptional modification that significantly influences gene and protein expression. It is involved in the regulation of various diseases, including those with an inflammatory component. This study investigates the potential role of m6A-mediated TREM-1 expression in the development of periodontitis. Clinical features and TREM-1 expression were assessed in periodontitis patients and healthy controls. LPS-stimulated human gingival fibroblasts (HGFs) were used to investigate m6A levels, m6A regulator METTL3, TREM-1, and inflammatory gene expression. In silico functional analysis explored TREM-1 interactions and functionalities. Periodontitis patients showed significantly elevated TREM-1 expression at both mRNA and protein levels. Predicted m6A motifs were present within the TREM-1 transcript. LPS stimulation of HGFs increased m6A content, METTL3, and TREM-1 expression, suggesting a potential link between m6A modification and TREM-1 regulation. Bioinformatic analysis revealed TREM-1 interaction with genes associated with periodontitis and its association with inflammatory pathways. This study suggests a potential role for METTL3-mediated m6A modification in regulating TREM-1 expression in periodontitis. Further investigation is needed to solidify this link and translate findings into clinical applications for improved periodontal health.
引用
收藏
页码:834 / 843
页数:10
相关论文
共 50 条
  • [1] N6-methyladenosine-mediated gene regulation and therapeutic implications
    Liu, Yujiao
    Yang, Dan
    Liu, Tao
    Chen, Jianjun
    Yu, Jianhua
    Yi, Ping
    TRENDS IN MOLECULAR MEDICINE, 2023, 29 (06) : 454 - 467
  • [2] Making Changes: N6-Methyladenosine-Mediated Decay Drives the Endothelial-to-Hematopoietic Transition
    Hsu, Phillip J.
    He, Chuan
    BIOCHEMISTRY, 2017, 56 (46) : 6077 - 6078
  • [3] Identification of an N6-methyladenosine-mediated positive feedback loop that promotes Epstein-Barr virus infection
    Dai, Dan-Ling
    Li, Xingyang
    Wang, Lin
    Xie, Chu
    Jin, Yanan
    Zeng, Mu-Sheng
    Zuo, Zhixiang
    Xia, Tian-Liang
    Journal of Biological Chemistry, 2021, 296
  • [4] The N6-methyladenosine-mediated lncRNA WEE2-AS1 promotes glioblastoma progression by stabilizing RPN2
    Li, Boyan
    Zhao, Rongrong
    Qiu, Wei
    Pan, Ziwen
    Zhao, Shulin
    Qi, Yanhua
    Qiu, Jiawei
    Zhang, Shouji
    Guo, Qindong
    Fan, Yang
    Xu, Hao
    Li, Ming
    Li, Gang
    Xue, Hao
    THERANOSTICS, 2022, 12 (14): : 6363 - 6379
  • [5] Identification of an N6-methyladenosine-mediated positive feedback loop that promotes Epstein-Barr virus infection
    Dai, Dan-Ling
    Li, Xingyang
    Wang, Lin
    Xie, Chu
    Jin, Yanan
    Zeng, Mu-Sheng
    Zuo, Zhixiang
    Xia, Tian-Liang
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2021, 296
  • [6] Elevated serum TREM-1 is associated with periodontitis and disease activity in rheumatoid arthritis
    Nevsun Inanc
    Gonca Mumcu
    Meryem Can
    Meral Yay
    Angelika Silbereisen
    Daniel Manoil
    Haner Direskeneli
    Nagihan Bostanci
    Scientific Reports, 11
  • [7] N6-methyladenosine-mediated LDHA induction potentiates chemoresistance of colorectal cancer cells through metabolic reprogramming
    Zhang, Kun
    Zhang, Tao
    Yang, Yuhan
    Tu, Wenling
    Huang, Hongbin
    Wang, Yujun
    Chen, Yuzhuo
    Pan, Kejian
    Chen, Zhuojia
    THERANOSTICS, 2022, 12 (10): : 4802 - 4817
  • [8] Ibrutinib Abrogates TREM-1 Mediated Neutrophil Activation
    Stadler, Nicole
    Hasibeder, Astrid
    Lopez, Pamela Aranda
    Desuki, Alexander
    Kriege, Oliver
    Weber, Alexander N. R.
    Michel, Christian
    Teschner, Daniel
    Hess, Georg
    Theobald, Matthias
    Radsak, Markus
    BLOOD, 2016, 128 (22)
  • [9] Elevated serum TREM-1 is associated with periodontitis and disease activity in rheumatoid arthritis
    Inanc, Nevsun
    Mumcu, Gonca
    Can, Meryem
    Yay, Meral
    Silbereisen, Angelika
    Manoil, Daniel
    Direskeneli, Haner
    Bostanci, Nagihan
    SCIENTIFIC REPORTS, 2021, 11 (01)
  • [10] N6-methyladenosine-mediated upregulation of LNCAROD confers radioresistance in esophageal squamous cell carcinoma through stabilizing PARP1
    Shi, Xiaobo
    Zhang, Xiaozhi
    Huang, Xinran
    Zhang, Ruijuan
    Pan, Shupei
    Huang, Shan
    Wang, Yuchen
    Ke, Yue
    Guo, Wei
    Liu, Xiaoxiao
    Hao, Yu
    Li, You
    Zhao, Xu
    Sun, Yuchen
    Li, Jing
    Ma, Hongbing
    Zhao, Xixi
    CLINICAL AND TRANSLATIONAL MEDICINE, 2024, 14 (10):