Generation and characterization of giant panda induced pluripotent stem cells

被引：3

作者：

Liu, Yuliang ^{[1
,2
]}

Zhang, Shihao ^{[3
,4
]}

Zou, Gaoyang ^{[3
,5
]}

An, Junhui ^{[1
,2
]}

Li, Yuan ^{[1
,2
]}

Lin, Danni ^{[3
]}

Wang, Donghui ^{[1
,2
]}

Li, Yan ^{[1
,2
]}

Chen, Jiasong ^{[1
,2
]}

Feng, Tongying ^{[1
,2
]}

Li, Hongyan ^{[1
,2
]}

Chen, Yijiao ^{[1
,2
]}

Zhang, Mingyue ^{[1
,2
]}

Kumar, Manish ^{[3
]}

Wang, Luqin ^{[3
]}

Hou, Rong ^{[1
,2
]}

Liu, Jing ^{[3
,5
,6
]}

机构：

[1] Chengdu Res Base Giant Panda Breeding, Chengdu 610081, Sichuan, Peoples R China

[2] Sichuan Key Lab Conservat Biol Endangered Wildlife, Chengdu 610081, Sichuan, Peoples R China

[3] Chinese Acad Sci, CAS Key Lab Regenerat Biol, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangdong Hong Kong Joint Lab Stem Cell & Regenera, Guangzhou 510530, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[5] GIBH, Guangzhou Regenerat Med & Hlth Guangdong Lab, Guangzhou 510530, Peoples R China

[6] Chinese Acad Sci, Hong Kong Inst Sci & Innovat, Ctr Regenerat Med & Hlth, Hong Kong, Peoples R China

来源：

SCIENCE ADVANCES | 2024年 / 10卷 / 38期

基金：

中国国家自然科学基金;

关键词：

X-CHROMOSOME INACTIVATION; SELF-RENEWAL; BONE-MARROW; FIBROBLASTS; INDUCTION; CULTURE; CANCER; NAIVE; LINES; IPSCS;

D O I：

10.1126/sciadv.adn7724

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The giant panda (Ailuropoda melanoleuca) stands as a flagship and umbrella species, symbolizing global biodiversity. While traditional assisted reproductive technology faces constraints in safeguarding the genetic diversity of giant pandas, induced pluripotent stem cells (iPSCs) known for their capacity to differentiate into diverse cells types, including germ cells, present a transformative potential for conservation of endangered animals. In this study, primary fibroblast cells were isolated from the giant panda, and giant panda iPSCs (GPiPSCs) were generated using a non-integrating episomal vector reprogramming method. Characterization of these GPiPSCs revealed their state of primed pluripotency and demonstrated their potential for differentiation. Furthermore, we innovatively formulated a species-specific chemically defined FACL medium and unraveled the intricate signaling pathway networks responsible for maintaining the pluripotency and fostering cell proliferation of GPiPSCs. This study provides key insights into rare species iPSCs, offering materials for panda characteristics research and laying the groundwork for in vitro giant panda gamete generation, potentially aiding endangered species conservation.

引用

页数：14

共 68 条

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