Chemical structure and immunomodulatory activity of a polysaccharide from Saposhnikoviae Radix

被引：3
作者：
He, Xinyang [1 ]
Fan, Haitao [1 ,2 ]
Sun, Meng [1 ]
Li, Jie [1 ]
Xia, Qing [3 ]
Jiang, Yanyan [1 ,4 ]
Liu, Bin [1 ,4 ]
机构：
[1] Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 102488, Peoples R China
[2] Beijing Polytech, Coll Bioengn, Beijing 100029, Peoples R China
[3] Qilu Univ Technol, Biol Inst, Shandong Acad Sci, Jinan 250103, Peoples R China
[4] State Adm Tradit Chinese Med, Key Lab Discovery Effect Subst Class Prescript Tra, Beijing 102488, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 276卷
基金：
中国国家自然科学基金;
关键词：
Saposhnikoviae Radix; Polysaccharide; Chemical structure; Immunomodulatory activity; Zebrafish experiment; ANTIOXIDANT ACTIVITY; PHYSICOCHEMICAL PROPERTIES; IN-VITRO; PURIFICATION; ZEBRAFISH; EXTRACTION; SULFATE;
D O I：
10.1016/j.ijbiomac.2024.133459
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new polysaccharide, named SP40015A01, was obtained from Saposhnikoviae Radix by water extraction, isolation and purification. SP40015A01 (9.7 x 105 Da) is composed of Rhamnose (Rha), Galacturonic acid (GalA), Galactose (Gal), and Arabinose (Ara) with the proportion of 1.6:85.6:5.8:7.6. The backbone of SP40015A01 is composed of 3-alpha-GalAp, 2-alpha-GalAp, 2,3-0-GalAp and 2,3-0-Galp, and branched at C3 of 2,30-GalAp, C3 of 2,3-0-Galp. Zebrafish experiments were used to explore the immunomodulatory activity of SP40015A01. Results showed that SP40015A01 could significantly improve the neutrophils density of immunocompromised zebrafish and reduce the content of nitric oxide (NO) and interleukin-1 beta (IL-1 beta). This study demonstrated that SP40015A01 has significant immunomodulatory activity, which can improve the neutrophils density and reduce inflammatory factor content, suggesting SP40015A01 may be a potential immunomodulator in Saposhnikoviae Radix (SR) for treatment of hypoimmunity related disease. This study supplemented the research on the polysaccharide components in traditional Chinese medicine and provided a scientific explanation for the development and clinical applications of SR.
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共 49 条
[1]   Extraction, purification, physicochemical properties and antioxidant activity of a new polysaccharide from Ocimum album L. seed [J].
Arab, Khaled ;
Ghanbarzadeh, Babak ;
Ayaseh, Ali ;
Jahanbin, Kambiz .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2021, 180 :643-653
[2]   A new water-soluble polysaccharide from Echinops pungens Trautv roots. Part I. Isolation, purification, characterization and antioxidant activity [J].
Benvidi, Seyedeh Mahsa Hoseiniyan ;
Jahanbin, Kambiz .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 161 :909-916
[3]   Novel polysaccharide from Chaenomeles speciosa seeds: Structural characterization, α-amylase and α-glucosidase inhibitory activity evaluation [J].
Deng, Yejun ;
Huang, Lixin ;
Zhang, Caihong ;
Xie, Pujun ;
Cheng, Jiang ;
Wang, Xiang ;
Liu, Lujie .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 153 :755-766
[4]   Structural characterization of polysaccharides from Saposhnikovia divaricata and their antagonistic effects against the immunosuppression by the culture supernatants of melanoma cells on RAW264.7 macrophages [J].
Dong, Cai-Xia ;
Liu, Liu ;
Wang, Chang-Ying ;
Fu, Zheng ;
Zhang, Ying ;
Hou, Xuan ;
Peng, Chao ;
Ran, Rui-Xue ;
Yao, Zhi .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 113 :748-756
[5]   Extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp [J].
Du, Hongtao ;
Chen, Juncheng ;
Tian, Shan ;
Gu, Hongling ;
Li, Na ;
Sun, Yao ;
Ru, Jiajia ;
Wang, Junru .
CARBOHYDRATE POLYMERS, 2016, 151 :348-357
[6]   Structure characterization and immunomodulatory activity of a polysaccharide from Saposhnikoviae Radix [J].
Fan, Haitao ;
Sun, Meng ;
Li, Jie ;
Zhang, Shuofeng ;
Tu, Guangzhong ;
Liu, Kechun ;
Xia, Qing ;
Jiang, Yanyan ;
Liu, Bin .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2023, 233
[7]   Structural characterization of polysaccharide from yellow sweet potato and ameliorates DSS-induced mice colitis by active GPR41/MEK/ERK 1/2 signaling pathway [J].
Feng, Xiaojuan ;
Du, Chuan ;
Wang, Chunling .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2021, 192 :278-288
[8]   Physicochemical properties and biological activities of polysaccharides from Lycium barbarum prepared by fractional precipitation [J].
Gong, Guiping ;
Dang, Tiantian ;
Deng, Yangni ;
Han, Jianli ;
Zou, Zihua ;
Jing, Sun ;
Zhang, Ying ;
Liu, Qian ;
Huang, Linjuan ;
Wang, Zhongfu .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 109 :611-618
[9]   Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity [J].
Hashemifesharaki, Reza ;
Xanthakis, Epameinondas ;
Altintas, Zeynep ;
Guo, Ya ;
Gharibzahedi, Seyed Mohammad Taghi .
CARBOHYDRATE POLYMERS, 2020, 240
[10]   The zebrafish reference genome sequence and its relationship to the human genome [J].
Howe, Kerstin ;
Clark, Matthew D. ;
Torroja, Carlos F. ;
Torrance, James ;
Berthelot, Camille ;
Muffato, Matthieu ;
Collins, John E. ;
Humphray, Sean ;
McLaren, Karen ;
Matthews, Lucy ;
McLaren, Stuart ;
Sealy, Ian ;
Caccamo, Mario ;
Churcher, Carol ;
Scott, Carol ;
Barrett, Jeffrey C. ;
Koch, Romke ;
Rauch, Gerd-Joerg ;
White, Simon ;
Chow, William ;
Kilian, Britt ;
Quintais, Leonor T. ;
Guerra-Assuncao, Jose A. ;
Zhou, Yi ;
Gu, Yong ;
Yen, Jennifer ;
Vogel, Jan-Hinnerk ;
Eyre, Tina ;
Redmond, Seth ;
Banerjee, Ruby ;
Chi, Jianxiang ;
Fu, Beiyuan ;
Langley, Elizabeth ;
Maguire, Sean F. ;
Laird, Gavin K. ;
Lloyd, David ;
Kenyon, Emma ;
Donaldson, Sarah ;
Sehra, Harminder ;
Almeida-King, Jeff ;
Loveland, Jane ;
Trevanion, Stephen ;
Jones, Matt ;
Quail, Mike ;
Willey, Dave ;
Hunt, Adrienne ;
Burton, John ;
Sims, Sarah ;
McLay, Kirsten ;
Plumb, Bob .
NATURE, 2013, 496 (7446) :498-503
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