A novel method to assess antibody-dependent cell-mediated cytotoxicity against influenza A virus M2 in immunized murine models

被引：0

作者：

Liang, Yinjie ^{[1
,2
]}

Guo, Junjia ^{[2
,3
]}

Li, Zhen ^{[1
,2
]}

Liu, Shiyuan ^{[2
,4
]}

Zhang, Ting ^{[1
]}

Sun, Shucai ^{[2
,5
]}

Lu, Funa ^{[2
,6
]}

Zhai, Yuqian ^{[2
]}

Wang, Wenling ^{[2
]}

Ning, Chuanyi ^{[1
]}

Tan, Wenjie ^{[2
,3
]}

机构：

[1] Guangxi Med Univ, Collaborat Innovat Ctr Regenerat Med & Med BioReso, Nanning 530021, Peoples R China

[2] Chinese Ctr Dis Control & Prevent, Natl Hlth Commiss Peoples Republ China, Natl Inst Viral Dis Control & Prevent, Key Lab Biosafety, Beijing 102206, Peoples R China

[3] Wenzhou Med Univ, Sch Lab Med, Wenzhou 325035, Peoples R China

[4] Baotou Med Coll, Sch Publ Hlth, Baotou 014040, Peoples R China

[5] Hebei Med Univ, Dept Nucl Med, Hosp 2, Shijiazhuang 050000, Peoples R China

[6] Inner Mongolia Med Univ, Sch Basic Med, Dept Microbiol, Hohhot 010110, Peoples R China

来源：

BIOSAFETY AND HEALTH | 2024年 / 6卷 / 03期

关键词：

Antibody-dependent cell-mediated; cytotoxicity (ADCC); Influenza A virus (IAV); Matrix protein 2 extracellular domain (M2e); Cell line; FC-GAMMA RECEPTORS; RELEASE; PROTEIN; ASSAY; DEGRANULATION;

D O I：

10.1016/j.bsheal.2024.04.002

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The matrix protein 2 (M2) is a preferred target for developing a universal vaccine against the influenza A virus (IAV). This study aimed to develop a method for assessing antibody-dependent cell-mediated cytotoxicity (ADCC) associated with M2-based immunization in mice. We first established a stable cell line derived from mouse lymphoma cells (YAC-1) expressing M2 of H3N2. This cell line, designated as YAC-1-M2, was generated using a second-generation lentiviral tricistronic plasmid system to transduce the M2 gene into YAC-1 cells. The ADCC effect induced by polyclonal antibodies targeting matrix protein 2 ectodomain (M2e) was demonstrated by YAC-1-M2 cell lysis by natural killer cells (NK) derived from mice, in the presence of anti-M2 antibodies obtained from mice immunized with an mRNA vaccine based on M2e. This ADCC effect was found to be stronger compared to the effect induced by monoclonal antibodies (14C2) against M2. Moreover, the ADCC effect was enhanced as the effector-to-target ratio of NK to YAC-1-M2 cells increased. In conclusion, we established a novel method to detect ADCC of M2 of IAV, which paves the way for the development of an M2-based universal vaccine against IAV and an in-depth analysis of its mechanism of broad-spectrum immune protection in mice. (c) 2024 Chinese Medical Association Publishing House. Published by Elsevier BV. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

引用

页码：178 / 185

页数：8

共 33 条

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