Simplified Synthesis of Poly(ethyleneimine)-Modified Silica Particles and Their Application in Oligosaccharide Isolation Methods

被引:2
|
作者
Zhao, Xingyun [1 ]
Niu, Yifan [1 ]
Zhao, Chengxiao [1 ]
Li, Zhenyu [1 ]
Li, Ke [1 ]
Qin, Xuemei [1 ]
机构
[1] Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Key Lab Effect Subst Res & Utilizat TCM Shanxi Pro, Key Lab Chem Biol & Mol Engn,Minist Educ, 92 Wucheng Rd, Taiyuan 030006, Peoples R China
基金
中国国家自然科学基金;
关键词
nucleophilic substitution; epoxy ring-opening reaction; HILIC stationary phase; polar compound; TCM oligosaccharides; STATIONARY-PHASE; ORDERED SILICA; CHROMATOGRAPHY;
D O I
10.3390/ijms25179465
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There are great challenges in the field of natural product isolation and purification and in the pharmacological study of oligosaccharide monomers. And these isolation and purification processes are still universal problems in the study of natural products (NPs), traditional Chinese medicine (TCM), omics, etc. The same polymer-modified materials designed for the special separation of oligosaccharides, named Sil-epoxy-PEI and Sil-chloropropyl-PEI, were synthesized via two different methods and characterized by scanning electron microscopy combined with energy spectrum analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, zeta potential as well as surface area analysis, etc. Several nucleotide/nucleoside molecules with different polarities and selectivities were successfully isolated in our laboratory using stainless-steel columns filled with the synthesized material. In addition, the separation of saccharide probes and oligosaccharides mixtures in water extracts of Morinda officinalis were compared in HILIC mode. The results showed that the resolution of separations for the representative analytes of the Sil-epoxy-PEI column was higher than for the Sil-chloropropyl-PEI column, and the developed stationary phase exhibited improved performance compared to hydrothermal carbon, amide columns and other HILIC materials previously reported.
引用
收藏
页数:11
相关论文
共 50 条
  • [21] Novel epoxy-silica hybrid coatings by using ethoxysilyl-modified hyperbranched poly(ethyleneimine) with improved scratch resistance
    Acebo, Cristina
    Fernandez-Francos, Xavier
    Messori, Massimo
    Ramis, Xavier
    Serra, Angels
    POLYMER, 2014, 55 (20) : 5028 - 5035
  • [22] Novel synthesis of poly(methyl methacrylate) brush encapsulated silica particles
    Ishizu, Koji
    Lee, Dong Hoon
    Tokuno, Yoko
    Uchida, Satoshi
    Ozawa, Masaaki
    Journal of Applied Polymer Science, 2008, 109 (06): : 3968 - 3974
  • [23] Novel synthesis of poly(methyl methacrylate) brush encapsulated silica particles
    Ishizu, Koji
    Lee, Dong Hoon
    Tokuno, Yoko
    Uchida, Satoshi
    Ozawa, Masaaki
    JOURNAL OF APPLIED POLYMER SCIENCE, 2008, 109 (06) : 3968 - 3974
  • [24] One-Pot and Rapid Synthesis of Uniformed Silica Spheres via Mediation of Linear Poly(ethyleneimine)s and Dyes
    Ren-Hua Jin
    Jian-Jun Yuan
    Polymer Journal, 2007, 39 : 822 - 827
  • [25] One-pot and rapid synthesis of uniformed silica spheres via mediation of linear poly(ethyleneimine)s and dyes
    Jin, Ren-Hua
    Yuan, Han-Jun
    POLYMER JOURNAL, 2007, 39 (08) : 822 - 827
  • [26] Synthesis and Structure of Composites of Nanosize Silica Particles Modified with Glass Microspheres
    V. M. Ogenko
    L. V. Dubrovina
    S. V. Volkov
    Russian Journal of Applied Chemistry, 2005, 78 : 197 - 199
  • [27] Synthesis and structure of composites of nanosize silica particles modified with glass microspheres
    Ogenko, VM
    Dubrovina, LV
    Volkov, SV
    RUSSIAN JOURNAL OF APPLIED CHEMISTRY, 2005, 78 (02) : 197 - 199
  • [28] The versatile surface properties of poly(cyclopentadiene)-modified silica particles (PCPD-silica): XPS and electrokinetic studies
    Simon, F
    Jacobasch, HJ
    Spange, S
    COLLOID AND POLYMER SCIENCE, 1998, 276 (10) : 930 - 939
  • [29] Synthesis of mesoporous silica modified with titania and application to gas adsorbent
    Enomoto, N
    Kawasaki, K
    Yoshida, M
    Li, XY
    Uehara, M
    Hojo, J
    SOLID STATE IONICS, 2002, 151 (1-4) : 171 - 175
  • [30] Poly(2-aminothiazole)-silica nanocomposite particles: Synthesis and morphology control
    Zou, Hua
    Wu, Di
    Sun, Hao
    Chen, Suwu
    Wang, Xia
    APPLIED SURFACE SCIENCE, 2018, 436 : 1083 - 1092