Simplified Synthesis of Poly(ethyleneimine)-Modified Silica Particles and Their Application in Oligosaccharide Isolation Methods

被引:2
|
作者
Zhao, Xingyun [1 ]
Niu, Yifan [1 ]
Zhao, Chengxiao [1 ]
Li, Zhenyu [1 ]
Li, Ke [1 ]
Qin, Xuemei [1 ]
机构
[1] Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Key Lab Effect Subst Res & Utilizat TCM Shanxi Pro, Key Lab Chem Biol & Mol Engn,Minist Educ, 92 Wucheng Rd, Taiyuan 030006, Peoples R China
基金
中国国家自然科学基金;
关键词
nucleophilic substitution; epoxy ring-opening reaction; HILIC stationary phase; polar compound; TCM oligosaccharides; STATIONARY-PHASE; ORDERED SILICA; CHROMATOGRAPHY;
D O I
10.3390/ijms25179465
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There are great challenges in the field of natural product isolation and purification and in the pharmacological study of oligosaccharide monomers. And these isolation and purification processes are still universal problems in the study of natural products (NPs), traditional Chinese medicine (TCM), omics, etc. The same polymer-modified materials designed for the special separation of oligosaccharides, named Sil-epoxy-PEI and Sil-chloropropyl-PEI, were synthesized via two different methods and characterized by scanning electron microscopy combined with energy spectrum analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, zeta potential as well as surface area analysis, etc. Several nucleotide/nucleoside molecules with different polarities and selectivities were successfully isolated in our laboratory using stainless-steel columns filled with the synthesized material. In addition, the separation of saccharide probes and oligosaccharides mixtures in water extracts of Morinda officinalis were compared in HILIC mode. The results showed that the resolution of separations for the representative analytes of the Sil-epoxy-PEI column was higher than for the Sil-chloropropyl-PEI column, and the developed stationary phase exhibited improved performance compared to hydrothermal carbon, amide columns and other HILIC materials previously reported.
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页数:11
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