Genetic characterization of lactococcosis-causing bacteria isolated from Brazilian native fish species

被引:2
|
作者
Barbanti, Angelo Carlo Chaparro [1 ]
do Rosario, Angelica Emanuely Costa [1 ]
Maia, Cynthia Rafaela Monteiro da Silva [1 ]
Rocha, victoria Pontes [2 ]
Costa, Henrique Lopes [2 ]
Trindade, Julia Miranda [2 ]
Nogueira, Luiz Fagner Ferreira [2 ]
Rosa, Julio Cesar Camara [2 ]
Ranzani-Paiva, Maria Jose Tavares [3 ]
Pilarski, Fabiana [4 ]
Gallani, Silvia Umeda [1 ]
Leal, Carlos Augusto Gomes [2 ]
Figueiredo, Henrique cesar Pereira [2 ]
Tavares, Guilherme Campos [2 ]
机构
[1] Nilton Lins Univ, Postgrad Program Aquaculture, Manaus, AM, Brazil
[2] Fed Univ Minas Gerais UFMG, Sch Vet Med, Dept Prevent Vet Med, Belo Horizonte, MG, Brazil
[3] Aquaculture Res Ctr, Fisheries Inst, APTA, SAA, Sao Paulo, Brazil
[4] Sao Paulo State Univ Unesp, Aquaculture Ctr Unesp, Lab Microbiol & Parasitol Aquat Organisms, Jaboticabal, SP, Brazil
关键词
Fish; Lactococcosis; Identification; Serotyping; Genotyping; FARMED RAINBOW-TROUT; GARVIEAE SEROTYPE II; ONCORHYNCHUS-MYKISS; NILE TILAPIA; STRAINS; PCR; PATHOGEN; IDENTIFICATION; CLONALITY; DIVERSITY;
D O I
10.1016/j.aquaculture.2024.741305
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Piscine lactococcosis is a disease caused by Lactococcus garvieae, L. petauri and L. formosensis. It is considered to be an emerging disease and poses a risk to global aquaculture production. Studies of the genetic diversity of Lactococcus spp. strains have epidemiological importance since they provide information on the genetic relationships between isolates and indicate geographic and temporal distribution. The aim of this study was to evaluate the genetic diversity of Brazilian Lactococcus spp. isolates obtained from native fish species using PCR-based DNA fingerprinting techniques. For this, 36 isolates were evaluated by gyrB sequencing, molecular serotyping, REP-, BOX-, and RAPD-PCR approaches. The discriminatory power and the congruence between the typing methods were calculated. After the correct taxonomic identification of the isolate, a total of 14, 5 and 17 isolates were reclassified as L. garvieae, L. formosensis and L. petauri, respectively. Lactococcus garvieae and L. petauri are related to serotype I, while L. formosensis strains were classified as serotype II. The typing methods tested are useful for evaluating the genetic diversity of isolates and a comprehensive indication of the diversity was found. Heterogeneous and homogeneous populations were observed for L. garvieae and L. petauri, respectively. Among the genotyping methods evaluated, RAPD-PCR demonstrated a greater discriminatory power for L. formosensis and L. petauri, while REP-PCR showed better results for L. garvieae strains, thus these methodologies are recommended for genotyping these bacterial species. In conclusion, Brazilian isolates of Lactococcus spp. obtained from native fish species constitute a genetically diverse population and the techniques used in this study allowed a better genetic characterization of the isolates.
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页数:11
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