Hydrogen Bond Strengthens Acceptor Group: The Curious Case of the C-H<middle dot><middle dot><middle dot>O=C Bond

An H-bond involves the sharing of a hydrogen atom between an electronegative atom to which it is covalently bound (the donor) and another electronegative atom serving as an acceptor. Such bonds represent a critically important geometrical force in biological macromolecules and, as such, have been characterized extensively. H-bond formation invariably leads to a weakening within the acceptor moiety due to the pulling exerted by the donor hydrogen. This phenomenon can be compared to a spring connecting two masses; pulling one mass stretches the spring, similarly affecting the bond between the two masses. Herein, we describe the opposite phenomenon when investigating the energetics of the C-H<middle dot><middle dot><middle dot>O=C bond. This bond underpins the most prevalent protein transmembrane dimerization motif (GxxxG) in which a glycine C alpha-H on one helix forms a hydrogen bond with a carbonyl in a nearby helix. We use isotope-edited FT-IR spectroscopy and corroborating computational approaches to demonstrate a surprising strengthening of the acceptor C=O bond upon binding with the glycine C alpha-H. We show that electronic factors associated with the C alpha-H bond strengthen the C=O oscillator by increasing the s-character of the sigma-bond, lowering the hyperconjugative disruption of the pi-bond. In addition, a reduction of the acceptor C=O bond's polarity is observed upon the formation of the C-H<middle dot><middle dot><middle dot>O=C bond. Our findings challenge the conventional understanding of H-bond dynamics and provide new insights into the structural stability of inter-helical protein interactions.