Identification and Quantification of Monosaccharides from Fungal Cell Walls and Exopolysaccharides by Gas Chromatography Coupled to Mass Spectrometry

被引：2

作者：

Millette, Pierre-Guy ^{[1
,2
,3
,5
]}

Chabot, Josee ^{[2
,3
]}

Sheppard, Donald C. ^{[1
,2
,3
,4
]}

Le Mauff, Francois ^{[2
,3
,4
]}

机构：

[1] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ, Canada

[2] McGill Univ, Res Inst, Hlth Ctr, Infect Dis & Immun Global Hlth Program, Montreal, PQ, Canada

[3] McGill Interdisciplinary Initiat Infect & Immun, Montreal, PQ, Canada

[4] Glyco NET Integrated Serv, Microbial Glyc Node, Montreal, PQ, Canada

[5] Univ Montreal, Fac Med, Montreal, PQ, Canada

来源：

CURRENT PROTOCOLS | 2023年 / 3卷 / 08期

关键词：

biofilm; cell wall; exopolysaccharide; GC-MS; monosaccharide;

D O I：

10.1002/cpz1.853

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The fungal cell wall and secreted exopolysaccharides play an important role in the interactions between fungi and their environment. Despite their central role in fungal biology, ecology, and host-pathogen interactions, the composition of these polymers and their synthetic pathways are not well understood. The protocols presented in this article describe an approach to isolate fungal cell wall polysaccharides and to identify and quantify the monosaccharide composition of these polymers by gas chromatography-mass spectrometry (GC-MS). (c) 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol: O-methyl trimethylsilyl monosaccharide derivatives composition analysis by GC-MSSupport Protocol: Fungal cell wall extraction

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页数：10

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