Synaptotagmin-9 in mouse retina

被引:0
作者
Mesnard, Chris S. [1 ,2 ,3 ]
Hays, Cassandra L. [1 ,2 ,4 ]
Townsend, Lou E. [1 ,2 ,3 ]
Barta, Cody L. [1 ,2 ]
Gurumurthy, Channabasavaiah B. [5 ]
Thoreson, Wallace B. [1 ,2 ,3 ]
机构
[1] Univ Nebraska, Truhlsen Eye Inst, Med Ctr, Omaha, NE 68182 USA
[2] Univ Nebraska, Med Ctr, Dept Ophthalmol & Visual Sci, Omaha, NE 68182 USA
[3] Univ Nebraska, Med Ctr, Pharmacol & Expt Neurosci, Omaha, NE 68182 USA
[4] Creighton Univ, Dept Med Educ, Omaha, NE USA
[5] Univ Nebraska, Med Ctr, Dept Genet Cell Biol & Anat, Omaha, NE USA
关键词
synaptotagmin; electroretinogram; rod photoreceptor cell; retina; ribbon synapse; TRANSPORTER-LIKE PROPERTIES; GLUTAMATE-TRANSPORTER; CONE PHOTORECEPTORS; EXPRESSION; GABA; RELEASE; IX; ELECTRORETINOGRAM; RECEPTORS; SENSORS;
D O I
10.1017/S0952523824000026
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Synaptotagmin-9 (Syt9) is a Ca2+ sensor mediating fast synaptic release expressed in various parts of the brain. The presence and role of Syt9 in retina is unknown. We found evidence for Syt9 expression throughout the retina and created mice to conditionally eliminate Syt9 in a cre-dependent manner. We crossed Syt9fl/fl mice with Rho-iCre, HRGP-Cre, and CMV-Cre mice to generate mice in which Syt9 was eliminated from rods (rodSyt9CKO), cones (coneSyt9CKO), or whole animals (CMVSyt9). CMVSyt9 mice showed an increase in scotopic electroretinogram (ERG) b-waves evoked by bright flashes with no change in a-waves. Cone-driven photopic ERG b-waves were not significantly different in CMVSyt9 knockout mice and selective elimination of Syt9 from cones had no effect on ERGs. However, selective elimination from rods decreased scotopic and photopic b-waves as well as oscillatory potentials. These changes occurred only with bright flashes where cone responses contribute. Synaptic release was measured in individual rods by recording anion currents activated by glutamate binding to presynaptic glutamate transporters. Loss of Syt9 from rods had no effect on spontaneous or depolarization-evoked release. Our data show that Syt9 acts at multiple sites in the retina and suggest that it may play a role in regulating transmission of cone signals by rods.
引用
收藏
页数:10
相关论文
共 46 条
  • [1] The role of GABA in modulating the Xenopus electroretinogram
    Arnarsson, A
    Eysteinsson, T
    [J]. VISUAL NEUROSCIENCE, 1997, 14 (06) : 1143 - 1152
  • [2] Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance
    Arriza, JL
    Eliasof, S
    Kavanaugh, MP
    Amara, SG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (08) : 4155 - 4160
  • [3] Multiple pathways of inhibition shape bipolar cell responses in the retina
    Eggers, Erika D.
    Lukasiewicz, Peter D.
    [J]. VISUAL NEUROSCIENCE, 2011, 28 (01) : 95 - 108
  • [4] Retinal glial cell glutamate transporter is coupled to an anionic conductance
    Eliasof, S
    Jahr, CE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) : 4153 - 4158
  • [5] Eliasof S, 1998, J NEUROSCI, V18, P698
  • [6] Fain Gordon, 2018, F1000Res, V7, DOI 10.12688/f1000research.14412.1
  • [7] Distinct developmental expression of synaptotagmin I and IX in the mouse brain
    Fukuda, M
    [J]. NEUROREPORT, 2006, 17 (02) : 179 - 182
  • [8] Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells
    Fukuda, M
    Kowalchyk, JA
    Zhang, XD
    Martin, TFJ
    Mikoshiba, K
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (07) : 4601 - 4604
  • [9] Fukuda M., 2008, Calcium Bind Proteins, V3, P1
  • [10] SYNAPTOTAGMIN-I - A MAJOR CA2+ SENSOR FOR TRANSMITTER RELEASE AT A CENTRAL SYNAPSE
    GEPPERT, M
    GODA, Y
    HAMMER, RE
    LI, C
    ROSAHL, TW
    STEVENS, CF
    SUDHOF, TC
    [J]. CELL, 1994, 79 (04) : 717 - 727