Wnt5a attenuates Wnt/β-catenin signalling in human dermal papilla cells

被引：29

作者：

Kwack, Mi Hee ^{[1
]}

Kim, Moon Kyu ^{[1
]}

Kim, Jung Chul ^{[1
]}

Sung, Young Kwan ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Dept Immunol, Sch Med, Taegu 700422, South Korea

来源：

EXPERIMENTAL DERMATOLOGY | 2013年 / 22卷 / 03期

关键词：

autocrine factor; dermal papilla; hair growth; Wnt/beta-catenin signalling; Wnt5a; HAIR SHAFT GROWTH; FOLLICLES; IDENTIFICATION; MORPHOGENESIS; EXPRESSION; RECEPTORS; WNT-B-10; PATHWAY; TARGET; SKIN;

D O I：

10.1111/exd.12101

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Findings of recent studies have demonstrated modulation of Wnt/beta-catenin signalling by Wnt5a, which is highly expressed in hair follicular dermal papilla (DP) in vivo. Here, we investigated the question of whether Wnt5a can affect canonical Wnt/beta-catenin signalling in DP cells. Treatment with Wnt5a resulted in attenuation of Wnt3a-mediated elevation of beta-catenin signalling, which was increased by Wnt5a siRNA transfection in cultured DP cells, as examined by reporter assay. In addition, treatment with Wnt5a resulted in repressed Wnt3a-mediated expression of Axin2, EP2 and LEF1 in cultured DP cells, whereas Wnt5a siRNA transfection resulted in increased Wnt3a-mediated expression of the genes in isolated DPs of cultured hair follicles. Moreover, treatment with Wnt5a resulted in attenuation of Wnt3a-mediated accumulation of beta-catenin in the nucleus in DP cells. Our data strongly suggest that Wnt5a acts as an autocrine factor and attenuates canonical Wnt signalling pathway in human DP cells.

引用

页码：229 / 231

页数：3

共 26 条

[1] Molecular control of epithelial-mesenchymal interactions during hair follicle cycling [J].