Dietary intake of sulforaphane-rich broccoli sprouts decreases fecal calprotectin levels in patients with ulcerative colitis

被引:0
作者
Yanaka, Akinori [1 ,2 ]
Ohmori, Toshihide [3 ]
Ochi, Masanori [4 ]
Suzuki, Hideo [5 ]
机构
[1] Univ Tsukuba, Dept Gastroenterol, Tsukuba, Ibaraki 3058575, Japan
[2] Tsukuba Gakuen Hosp, Dept Gastroenterol, Tsukuba, Ibaraki 3050854, Japan
[3] Ohmori Toshihide Gastrointestinal Clin, Ageo 3620075, Japan
[4] Hitachi Gen Hosp, Dept Radiol, Hitachi, Ibaraki 3170077, Japan
[5] Tsukuba Coll Technol Clin, Tsukuba, Ibaraki 3050821, Japan
来源
FUNCTIONAL FOODS IN HEALTH AND DISEASE | 2024年 / 14卷 / 10期
基金
日本学术振兴会;
关键词
D O I
10.31989/ffhd.v14i10.1440
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Background: Ulcerative colitis (UC), which is linked to chronic oxidative stress, has been on the rise in Japan, attributed to the recent Westernization of dietary habits. Sulforaphane, which is abundant in broccoli sprouts (BS), has been shown to enhance antioxidant activity by up-regulating nrf2-mediated antioxidant enzymes. We have previously shown that the dietary intake of sulforaphane-rich broccoli sprouts mitigates H.pylori-induced gastritis not only by enhancing nrf2dependent anti-oxidant activity against oxidative stress from H.pylori infection, but also by directly inhibiting H.pylori activity (Cancer Prev Res 2:353-360,2009). In this study, we examined if the dietary approach with BS affects intestinal microbiota, thereby mitigating colonic inflammation in mesalazine-treated human UC patients. Objective: In this study, we examined if dietary intake of sulforaphane-rich broccoli sprouts decreases fecal calprotectin levels in patients with ulcerative colitis Methods: This study was registered with the University hospital Medical Information Network (UMIN) in Japan under the Clinical Trial Registration Number, UMIN000041972. Twenty-eight mild UC patients treated with mesalazine were divided between the sulforaphane-rich BS group (n=14) or the sulforaphane-free alfalfa sprouts (AS) group (n=14). These subjects were instructed to take 20 g of raw BS or AS daily for 8 weeks, with BS containing 4.4 mg/g glucoraphanin, a precursor of sulforaphane, and AS containing no glucoraphanin. Stool samples were obtained just before and after the 8 weeks sprouts treatment. Levels of fecal calprotectin were measured as the quantitative indices for colonic mucosal inflammation. We conducted an examination of gut bacteria through a method called terminal restriction fragment length polymorphism (TR-FLP) analysis of fecal samples. Results: 1. Twelve patients from the BS group and 11 from the AS group completed the intervention study as requested. 2. Only the treatment with BS caused a significant decrease in the level of fecal calprotectin as well as an increase in the fecal component of Clostridium subcluster IV and XIVa, which have been reported to enhance butyrate production. 3. There was no change in the clinical activity index after intake of either the BS or the AS. Conclusion: These results indicate that dietary approach with sulforaphane-rich BS mitigates colonic inflammation in mesalazine-treated UC patients. Our data also suggest that the beneficial effects of the BS may be related with the increase in butyrate-producing intestinal microbiota by dietary approach with sulforaphane.
引用
收藏
页码:688 / 703
页数:16
相关论文
共 29 条
[1]   Anticancer properties of sulforaphane: current insights at the molecular level [J].
Asif Ali, Muhammad ;
Khan, Noohela ;
Kaleem, Nabeeha ;
Ahmad, Waqas ;
Alharethi, Salem Hussain ;
Alharbi, Bandar ;
Alhassan, Hassan H. ;
Al-Enazi, Maher M. ;
Razis, Ahmad Faizal Abdull ;
Modu, Babagana ;
Calina, Daniela ;
Sharifi-Rad, Javad .
FRONTIERS IN ONCOLOGY, 2023, 13
[2]   Methods for non-proportional hazards in clinical trials: A systematic review [J].
Bardo, Maximilian ;
Huber, Cynthia ;
Benda, Norbert ;
Brugger, Jonas ;
Fellinger, Tobias ;
Galaune, Vaidotas ;
Heinz, Judith ;
Heinzl, Harald ;
Hooker, Andrew C. ;
Klinglmueller, Florian ;
Koenig, Franz ;
Mathes, Tim ;
Mittlboeck, Martina ;
Posch, Martin ;
Ristl, Robin ;
Friede, Tim .
STATISTICAL METHODS IN MEDICAL RESEARCH, 2024, 33 (06) :1069-1092
[3]   The interplay between cytokines, inflammation, and antioxidants: mechanistic insights and therapeutic potentials of various antioxidants and anti-cytokine compounds [J].
Bhol, Nitish Kumar ;
Bhanjadeo, Madhabi Madhusmita ;
Singh, Anup Kumar ;
Dash, Umesh Chandra ;
Ojha, Rakesh Ranjan ;
Majhi, Sanatan ;
Duttaroy, Asim K. ;
Jena, Atala Bihari .
BIOMEDICINE & PHARMACOTHERAPY, 2024, 178
[4]   Protective effects of sulforaphane against toxic substances and contaminants: A systematic review [J].
Cascajosa-Lira, Antonio ;
Prieto, Ana, I ;
Pichardo, Silvia ;
Jos, Angeles ;
Camean, Ana M. .
PHYTOMEDICINE, 2024, 130
[5]   Therapeutic advancement in inflammatory bowel disease by incorporating plant-based diet [J].
Chiba, Mitsuro ;
Tsuji, Tsuyotoshi ;
Komatsu, Masafumi .
TRANSLATIONAL GASTROENTEROLOGY AND HEPATOLOGY, 2023, 8
[6]   Management and treatment optimization of patients with mild to moderate ulcerative colitis [J].
D'Amico, Ferdinando ;
Fasulo, Ernesto ;
Jairath, Vipul ;
Paridaens, Kristine ;
Peyrin-Biroulet, Laurent ;
Danese, Silvio .
EXPERT REVIEW OF CLINICAL IMMUNOLOGY, 2024, 20 (03) :277-290
[7]   Influence of Source Materials, Concentration, Gastric Digestion, and Encapsulation on the Bioactive Response of Brassicaceae-Derived Samples against Helicobacter pylori [J].
Garcia-Ibanez, Paula ;
Silvan, Jose Manuel ;
Moreno, Diego A. ;
Carvajal, Micaela ;
Martinez-Rodriguez, Adolfo J. .
MICROORGANISMS, 2024, 12 (01)
[8]   The Protective Effect of Sulforaphane on Dextran Sulfate Sodium-Induced Colitis Depends on Gut Microbial and Nrf2-Related Mechanism [J].
He, Canxia ;
Gao, Mingfei ;
Zhang, Xiaohong ;
Lei, Peng ;
Yang, Haitao ;
Qing, Yanping ;
Zhang, Lina .
FRONTIERS IN NUTRITION, 2022, 9
[9]   Comparison of fecal calprotectin levels and endoscopic scores for predicting relapse in patients with ulcerative colitis in remission [J].
Ishida, Natsuki ;
Ito, Tatsuhiro ;
Takahashi, Kenichi ;
Asai, Yusuke ;
Miyazu, Takahiro ;
Higuchi, Tomohiro ;
Tamura, Satoshi ;
Tani, Shinya ;
Yamade, Mihoko ;
Iwaizumi, Moriya ;
Hamaya, Yasushi ;
Osawa, Satoshi ;
Sugimoto, Ken .
WORLD JOURNAL OF GASTROENTEROLOGY, 2023, 29 (47) :6111-6121
[10]   Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts [J].
Kasamatsu, Shingo ;
Owaki, Takuma ;
Komae, Somei ;
Kinno, Ayaka ;
Ida, Tomoaki ;
Akaike, Takaaki ;
Ihara, Hideshi .
REDOX BIOLOGY, 2023, 67