A high-throughput cell-based screening method for Zika virus protease inhibitor discovery

被引:3
作者
Anindita, Paulina Duhita [1 ,2 ]
Otsuka, Yuka [3 ]
Lattmann, Simon [2 ]
Ngo, Khac Huy [1 ,2 ]
Liew, Chong Wai [2 ]
Kang, Congbao [4 ]
Harris, Reuben S. [5 ,6 ]
Scampavia, Louis [3 ]
Spicer, Timothy P. [3 ]
Luo, Dahai [1 ,2 ,7 ]
机构
[1] Nanyang Technol Univ, Lee Kong Chian Sch Med, Singapore, Singapore
[2] Nanyang Technol Univ, NTU Inst Struct Biol, Singapore, Singapore
[3] Herbert Wertheim UF Scripps Inst Biomed Innovat &, Dept Mol Med, Jupiter, FL 33458 USA
[4] ASTAR, Expt Drug Dev Ctr, Singapore, Singapore
[5] Univ Texas Hlth San Antonio, Dept Biochem & Struct Biol, San Antonio, TX USA
[6] Univ Texas Hlth San Antonio, Howard Hughes Med Inst, San Antonio, TX USA
[7] Natl Ctr Infect Dis, Singapore, Singapore
关键词
Zika virus; HTS; Cell-based assay; Protease inhibitors;
D O I
10.1016/j.slasd.2024.100164
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Zika virus (ZIKV) continues to pose a significant global public health threat, with recurring regional outbreaks and potential for pandemic spread. Despite often being asymptomatic, ZIKV infections can have severe consequences, including neurological disorders and congenital abnormalities. Unfortunately, there are currently no approved vaccines or antiviral drugs for the prevention or treatment of ZIKV. One promising target for drug development is the ZIKV NS2B-NS3 protease due to its crucial role in the virus life cycle. In this study, we established a cell-based ZIKV protease inhibition assay designed for high-throughput screening (HTS). Our assay relies on the ZIKV protease's ability to cleave a cyclised firefly luciferase fused to a natural cleavage sequence between NS2B and NS3 protease within living cells. We evaluated the performance of our assay in HTS setting using the pharmacologic controls (JNJ-40418677 and MK-591) and by screening a Library of Pharmacologically Active Compounds (LOPAC). The results confirmed the feasibility of our assay for compound library screening to identify potential ZIKV protease inhibitors.
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页数:9
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