Effects of Head-Only Exposure to 900 MHz GSM Electromagnetic Fields in Rats: Changes in Neuronal Activity as Revealed by c-Fos Imaging without Concomitant Cognitive Impairments

被引:0
作者
Bontempi, Bruno [1 ,2 ]
Leveque, Philippe [3 ]
Dubreuil, Diane [4 ]
Jay, Therese M. [5 ]
Edeline, Jean-Marc [4 ]
机构
[1] Univ Bordeaux, CNRS, Inst Neurosci Cognit & Integrat Aquitaine, UMR 5287, F-33000 Bordeaux, France
[2] Ecole Prat Hautes Etud, F-33000 Bordeaux, France
[3] Univ Limoges, CNRS, XLIM, UMR 6172, F-87060 Limoges, France
[4] Univ Paris Saclay, CNRS, Inst Neurosci Paris Saclay NeuroPSI, CEA Paris Saclay, Bat 151, F-91400 Saclay, France
[5] Univ Paris Cite, Inst Psychiat & Neurosci Paris, INSERM, UMR S 1266, F-75014 Paris, France
关键词
spatial learning; immunohistochemistry; rat; radio frequency; COMMUNICATION MICROWAVE EXPOSURE; SPATIAL-LEARNING TASK; ARM MAZE PERFORMANCE; AFFECTS HUMAN SLEEP; HZ MAGNETIC-FIELD; CELLULAR PHONES; CONTINUOUS-WAVE; MOBILE PHONES; BRAIN; MEMORY;
D O I
10.3390/biomedicines12091954
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Over the last two decades, animal models have been used to evaluate the physiological and cognitive effects of mobile phone exposure. Here, we used a head-only exposure system in rats to determine whether exposure to 900 MHz GSM electromagnetic fields (EMFs) induces regional changes in neuronal activation as revealed by c-Fos imaging. In a first study, rats were exposed for 2 h to brain average specific absorption rates (BASARs) ranging from 0.5 to 6 W/kg. Changes in neuronal activation were found to be dose-dependent, with significant increases in c-Fos expression occurring at BASAR of 1 W/kg in prelimbic, infralimbic, frontal, and cingulate cortices. In a second study, rats were submitted to either a spatial working memory (WM) task in a radial maze or a spatial reference memory (RM) task in an open field arena. Exposures (45 min) were conducted before each daily training session (BASARs of 1 and 3.5 W/kg). Control groups included sham-exposed and control cage animals. In both tasks, behavioral performance evolved similarly in the four groups over testing days. However, c-Fos staining was significantly reduced in cortical areas (prelimbic, infralimbic, frontal, cingulate, and visual cortices) and in the hippocampus of animals engaged in the WM task (BASARs of 1 and 3.5 W/kg). In the RM task, EMF exposure-induced decreases were limited to temporal and visual cortices (BASAR of 1 W/kg). These results demonstrate that both acute and subchronic exposures to 900 MHz EMFs can produce region-specific changes in brain activity patterns, which are, however, insufficient to induce detectable cognitive deficits in the behavioral paradigms used here.
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