In the present work, Titanium oxide nanoparticles (TiO2 NPs) were synthesized by the solution combustion method for use in Riboflavin (RF) sensor applications. Titanium (III) sulphate was used as a precursor and glycine as a fuel. These reactants were mixed and heated at 800( degrees)C to produce TiO2 NPs. The crystal phase and surface topography of TiO2 NPs was studied by X-ray diffraction method (XRD) and Field emission scanning electron microscopy (FE-SEM). TiO(2 )composite carbon paste electrode (TiO2CCPE), and electrochemically polymerized aniline-modified TiO2 composite with carbon paste electrode (AN-MTiO2CCPE) were studied by Scanning electron microscopy (SEM). The composition of Ti and O of TiO2 NPs was determined by energy-dispersive X-ray spectroscopy (EDS). In Raman spectroscopy, the absorbance peaks of TiO2 NPs were studied. These prepared TiO2 NPs were used to develop the sensor for RF using electro-polymerization of aniline on the surface of the TiO2CCPE. The electrochemical analysis of the RF was studied using different parameters like the effect of pH on 0.2 M phosphate buffer solution (PBS) at 6.0 pH with scan rate variation ranges of 0.025-0.450 V/s, it shows an absorption-controlled reaction and concentration variation of RF from 0.2 mu M to 3.4 mu M by differential pulse voltammetry (DPV). The lower Limit of detection (LOD) and quantification (LOQ) of redox reaction were 0.28 mu M and 0.45 mu M respectively. The AN-MTIO2CCPE shows better stability, sensitivity, selectivity, and retentiveness, in RF detection. The analytical application of the Vitamin B12 tablet sample was also studied using a developed sensor. The modification of electrodes with the prepared TiO2 NPs helps in a better way of electrochemical sensing using voltammetric study with better sensitivity, selectivity, and reproducibility of riboflavin detection compared to commercially available nanoparticles.
