Synthesis of TiO2 nanoparticles for the detection of riboflavin by voltammetric studies

In the present work, Titanium oxide nanoparticles (TiO2 NPs) were synthesized by the solution combustion method for use in Riboflavin (RF) sensor applications. Titanium (III) sulphate was used as a precursor and glycine as a fuel. These reactants were mixed and heated at 800( degrees)C to produce TiO2 NPs. The crystal phase and surface topography of TiO2 NPs was studied by X-ray diffraction method (XRD) and Field emission scanning electron microscopy (FE-SEM). TiO(2 )composite carbon paste electrode (TiO2CCPE), and electrochemically polymerized aniline-modified TiO2 composite with carbon paste electrode (AN-MTiO2CCPE) were studied by Scanning electron microscopy (SEM). The composition of Ti and O of TiO2 NPs was determined by energy-dispersive X-ray spectroscopy (EDS). In Raman spectroscopy, the absorbance peaks of TiO2 NPs were studied. These prepared TiO2 NPs were used to develop the sensor for RF using electro-polymerization of aniline on the surface of the TiO2CCPE. The electrochemical analysis of the RF was studied using different parameters like the effect of pH on 0.2 M phosphate buffer solution (PBS) at 6.0 pH with scan rate variation ranges of 0.025-0.450 V/s, it shows an absorption-controlled reaction and concentration variation of RF from 0.2 mu M to 3.4 mu M by differential pulse voltammetry (DPV). The lower Limit of detection (LOD) and quantification (LOQ) of redox reaction were 0.28 mu M and 0.45 mu M respectively. The AN-MTIO2CCPE shows better stability, sensitivity, selectivity, and retentiveness, in RF detection. The analytical application of the Vitamin B12 tablet sample was also studied using a developed sensor. The modification of electrodes with the prepared TiO2 NPs helps in a better way of electrochemical sensing using voltammetric study with better sensitivity, selectivity, and reproducibility of riboflavin detection compared to commercially available nanoparticles.