Synovial mesenchymal stem cells secrete more lubricin than adipose mesenchymal stem cells after injection into rat osteoarthritis knees

被引:1
作者
Matsuta, Seiya [1 ]
Endo, Kentaro [1 ]
Ozeki, Nobutake [1 ]
Nakagawa, Yusuke [2 ]
Koga, Hideyuki [3 ]
Sekiya, Ichiro [1 ]
机构
[1] Tokyo Med & Dent Univ, Ctr Stem Cell & Regenerat Med, 1-5-45 Yushima,Bunkyo Ku, Tokyo 1138510, Japan
[2] Tokyo Med & Dent Univ, Dept Cartilage Regenerat, 1-5-45 Yushima,Bunkyo Ku, Tokyo 1138510, Japan
[3] Tokyo Med & Dent Univ, Dept Joint Surg & Sports Med, 1-5-45 Yushima,Bunkyo Ku, Tokyo 1138510, Japan
关键词
Mesenchymal stem cell; Osteoarthritis; Lubricin; Intra-articular injection; CARTILAGE; TISSUES;
D O I
10.1016/j.bbrc.2024.150354
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Intra-articular injection of mesenchymal stem cells (MSCs) is envisioned as a solution for knee osteoarthritis (OA). Although synovial MSCs (SyMSCs) are promising for cartilage regeneration, the clinical choice is usually adipose MSCs (AdMSCs). However, the similarities/differences in the mode of action between SyMSCs and AdMSCs remain unclear. Here, we compared factors secreted by human SyMSCs and AdMSCs after injection into OA knees. Human SyMSCs or AdMSCs were injected into the knees of rat partial meniscectomy models. The next day, the knee joints were collected to analyze the distribution of injected MSCs and transcriptome changes in the human MSCs and rat synovium. Non-injected MSCs were mixed with rat synovium as a control. After injection, no difference was apparent in intra-articular distribution of the SyMSCs or AdMSCs. RNA sequencing demonstrated an enrichment of cytokine-cytokine receptor interaction-related genes in both human SyMSCs and AdMSCs after injection. Differentially expressed genes (DEGs) specific to SyMSCs were associated with cartilage matrix synthesis and homeostasis. PCR analysis of the matrisome-related DEGs showed significantly higher expression of PRG4 in SyMSCs than in AdMSCs after injection. Immunostaining also confirmed a significantly greater expression of lubricin by SyMSCs than by AdMSCs. These findings indicate that SyMSCs will be a more promising treatment for OA.
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页数:7
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