Interactive effects of chlorothalonil and Varroa destructor on Apis mellifera during adult stage

被引:0
作者
Wu, Tong [1 ]
Choi, Yong Soo [2 ]
Kim, Dong Won [2 ]
Wei, Xiaoping [3 ]
Kang, Yuxin [1 ]
Han, Bo [1 ]
Yang, Sa [1 ]
Gao, Jing [1 ]
Dai, Pingli [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Apicultural Res, State Key Lab Resource Insects, Beijing 100193, Peoples R China
[2] Natl Inst Agr Sci, Rural Dev Adm, Dept Agr Biol, Wanju Gun 55365, South Korea
[3] Guizhou Acad Agr Sci, Modern Agr Dev Inst, Guiyang 550006, Peoples R China
基金
北京市自然科学基金;
关键词
Honey bee; Varroa destructor; Chlorothalonil; Survival; Fat body; BEE; PROTEIN; GENE; MITE; VITELLOGENIN; METABOLISM; PESTICIDES; PATHWAYS; IMMUNITY;
D O I
10.1016/j.pestbp.2024.106107
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The interaction between environmental factors affecting honey bees is of growing concern due to their potential synergistic effects on bee health. Our study investigated the interactive impact of Varroa destructor and chlorothalonil on workers' survival, fat body morphology, and the expression of gene associated with detoxification, immunity, and nutrition metabolism during their adult stage. We found that both chlorothalonil and V. destructor significantly decreased workers' survival rates, with a synergistic effect observed when bees were exposed to both stressors simultaneously. Morphological analysis of fat body revealed significant alterations in trophocytes, particularly a reduction in vacuoles and granules after Day 12, coinciding with the transition of the bees from nursing to other in-hive work tasks. Gene expression analysis showed significant changes in detoxification, immunity, and nutrition metabolism over time. Detoxification genes, such as CYP9Q2, , CYP9Q3, , and GST-D1, , were downregulated in response to stressor exposure, indicating a potential impairment in detoxification processes. Immune-related genes, including defensin-1, , Dorsal-1 , and Kayak, , exhibited an initially upregulation followed by varied expression patterns, suggesting a complex immune response to stressors. Nutrition metabolism genes, such as hex 70a, , AmIlp2, , VGMC, , AmFABP, , and AmPTL, , displayed dynamic expression changes, reflecting alterations in nutrient utilization and energy metabolism in response to stressors. Overall, these findings highlight the interactive and dynamic effects of environmental stressor on honey bees, providing insights into the mechanisms underlying honey bee decline. These results emphasize the need to consider the interactions between multiple stressors in honey bee research and to develop management strategies to mitigate their adverse effects on bee populations.
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页数:9
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共 92 条
  • [1] Hormonal control of the yolk precursor vitellogenin regulates immune function and longevity in honeybees
    Amdam, GV
    Simoes, ZLP
    Hagen, A
    Norberg, K
    Schroder, K
    Mikkelsen, O
    Kirkwood, TBL
    Omholt, SW
    [J]. EXPERIMENTAL GERONTOLOGY, 2004, 39 (05) : 767 - 773
  • [2] The hive bee to forager transition in honeybee colonies: the double repressor hypothesis
    Amdam, GV
    Omholt, SW
    [J]. JOURNAL OF THEORETICAL BIOLOGY, 2003, 223 (04) : 451 - 464
  • [3] Social exploitation of vitellogenin
    Amdam, GV
    Norberg, K
    Hagen, A
    Omholt, SW
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (04) : 1799 - 1802
  • [4] Transcriptional regulation of xenobiotic detoxification genes in insects-An overview
    Amezian, Dries
    Nauen, Ralf
    Le Goff, Gaelle
    [J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2021, 174
  • [5] Neonicotinoid Clothianidin reduces honey bee immune response and contributes to Varroa mite proliferation
    Annoscia, Desiderato
    Di Prisco, Gennaro
    Becchimanzi, Andrea
    Caprio, Emilio
    Frizzera, Davide
    Linguadoca, Alberto
    Nazzi, Francesco
    Pennacchio, Francesco
    [J]. NATURE COMMUNICATIONS, 2020, 11 (01)
  • [6] Haemolymph removal by Varroa mite destabilizes the dynamical interaction between immune effectors and virus in bees, as predicted by Volterra's model
    Annoscia, Desiderato
    Brown, Sam P.
    Di Prisco, Gennaro
    De Paoli, Emanuele
    Del Fabbro, Simone
    Frizzera, Davide
    Zanni, Virginia
    Galbraith, David A.
    Caprio, Emilio
    Grozinger, Christina M.
    Pennacchio, Francesco
    Nazzi, Francesco
    [J]. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2019, 286 (1901)
  • [7] Insect Fat Body: Energy, Metabolism, and Regulation
    Arrese, Estela L.
    Soulages, Jose L.
    [J]. ANNUAL REVIEW OF ENTOMOLOGY, 2010, 55 : 207 - 225
  • [8] Xenobiotic detoxification pathways in honey bees
    Berenbaum, May R.
    Johnson, Reed M.
    [J]. CURRENT OPINION IN INSECT SCIENCE, 2015, 10 : 51 - 58
  • [9] Parasites and pesticides act antagonistically on honey bee health
    Bird, Gwendolyn
    Wilson, Alan E.
    Williams, Geoffrey R.
    Hardy, Nate B.
    [J]. JOURNAL OF APPLIED ECOLOGY, 2021, 58 (05) : 997 - 1005
  • [10] Interaction between Varroa destructor and imidacloprid reduces flight capacity of honeybees
    Blanken, Lisa J.
    van Langevelde, Frank
    van Dooremalen, Coby
    [J]. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2015, 282 (1820)