In guava plants, nodal cuttings are the most efficient and effective way for propagation. Plant growth regulators that are appropriate for accelerating guava growth. To improve and stimulate the development of roots in guava nodal cuttings with the help of plant growth regulators. In this study, guava nodal cuttings were used at concentrations of 200 and 500 ppm, together with several kinds of cuttings, to examine the effects of three phytohormones, including cytokinin, indole-3-butyric acid ( IBA ), and indole-3-acetic acid ( IAA ) on single node, double node, triple node, and leaf cuts. Horticultural College and Research Institute, Periyakulam, Tamil Nadu was the site of the experiment. Nodal cuttings, double nodal cuttings, and IBA better performed the other plant growth regulators. The treatments with 500 ppm IBA in the double nodal cuttings resulted in the following: the lowest phenol content (0.030 mg/g), the lowest starch content (3.42 %), and the highest C/N ratio (8.66). The weight of the leaf was 1.73 g, the weight of the leaf after it had been dried was 0.68 g, the weight of the root (fresh) was 3.69 g, and the weight of the root (dry) was (1.86 g). With the longest root length and maximum chlorophyll content (41.94 mg/g) among all the cuttings treated with 500 ppm of IBA, the triple node cuttings stood out (27.32 cm). The cuttings collected from a single node and treated with 500 ppm IBA observed the highest amount of leaf-soluble protein at 38.92 (mg/g). It can be concluded that the combined effect of growth regulator IBA at 500 ppm double cuttings performed the best on the root and shoot parameters and also nutritional component and antioxidant activities compared to the other treatments.