Preparation of a novel type I feline coronavirus virus-like particle vaccine and its immunogenicity in mice and cats

被引：1

作者：

Zhou, Qun ^{[1
]}

Song, Xin ^{[1
]}

Li, Yan ^{[1
,2
]}

Huang, Jian ^{[1
,2
]}

Yu, Qi-sheng ^{[1
]}

Den, Gu-nan ^{[1
]}

Zhang, Jia-qi ^{[1
]}

Zhu, Chen-xi ^{[1
]}

Zhang, Bin ^{[1
,2
]}

机构：

[1] Southwest Minzu Univ, Coll Anim & Vet Sci, Chengdu 610041, Peoples R China

[2] Key Lab Minist Educ & Sichuan Prov Qinghai Tibetan, Chengdu 610041, Peoples R China

来源：

MICROBIAL PATHOGENESIS | 2024年 / 194卷

关键词：

Feline coronavirus; Virus -like particles; Vaccine; Immunogenicity; INFECTIOUS PERITONITIS; E-PROTEIN; DOMAIN; EFFICACY; ABSENCE; SAFETY;

D O I：

10.1016/j.micpath.2024.106795

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Feline coronavirus (FCoV) infection is a leading cause of death in cats. In this study, we produced FCoV-I viruslike particles (VLPs) containing E, M, N, and S proteins using a baculovirus expression system and mixed VLPs with the adjuvants MF59 and CpG 55.2 to prepare an VLP/MF59/CpG vaccine. After immunization of mice with the vaccine, IgG specific antibodies titers against S and N proteins increased to 1:12,800, and IFN-gamma+ and IL-4+ splenocytes were significantly increased. Following immunization of FCoV-negative cats, the S protein antibodies in immunized cats (5/5) increased significantly, with a peak of 1:12,800. Notably, after booster vaccination in FCoV-positive cats, a significant reduction in viral load was observed in the feces of partial cats (4/5), and the FCoV-I negative conversion was found in two immunized cats (2/5). Therefore, the VLP/MF59/CpG vaccine is a promising candidate vaccine to prevent the FCoV infection.

引用

页数：8

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