Transcriptomic Insights into Salt Stress Response in Two Pepper Species: The Role of MAPK and Plant Hormone Signaling Pathways

被引:5
|
作者
Farooq, Muhammad Aamir [1 ,2 ]
Ul Haq, Muhammad Zeeshan [1 ]
Zhang, Liping [1 ,2 ]
Wu, Shuhua [1 ,2 ]
Mushtaq, Naveed [1 ,2 ]
Tahir, Hassam [1 ,2 ]
Wang, Zhiwei [1 ,2 ]
机构
[1] Hainan Univ, Sanya Inst Breeding & Multiplicat, Key Lab Qual Regulat Trop Hort Crops Hainan Prov, Sch Breeding & Multiplicat,Ctr Nanfan & High Effic, Sanya 572025, Peoples R China
[2] Hainan Univ, Sch Trop Agr & Forestry, Key Lab Qual Regulat Trop Hort Crops Hainan Prov, Haikou 570228, Peoples R China
关键词
pepper; salt stress; transcriptome; WGCNA; enzymatic antioxidants; SALINITY STRESS; PROTEIN-KINASES; TOLERANCE; ABA; ARABIDOPSIS; IMPROVES; GENES;
D O I
10.3390/ijms25179355
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Salt stress imposes significant plant limitations, altering their molecular, physiological, and biochemical functions. Pepper, a valuable herbaceous plant species of the Solanaceae family, is particularly susceptible to salt stress. This study aimed to elucidate the physiological and molecular mechanisms that contribute to the development of salt tolerance in two pepper species (Capsicum baccatum (moderate salt tolerant) and Capsicum chinense (salt sensitive)) through a transcriptome and weighted gene co-expression network analysis (WGCNA) approach to provide detailed insights. A continuous increase in malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels in C. chinense and higher activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) in C. baccatum indicated more tissue damage in C. chinense than in C. baccatum. In transcriptome analysis, we identified 39 DEGs related to salt stress. Meanwhile, KEGG pathway analysis revealed enrichment of MAPK and hormone signaling pathways, with six DEGs each. Through WGCNA, the ME.red module was identified as positively correlated. Moreover, 10 genes, A-ARR (CQW23_24856), CHIb (CQW23_04881), ERF1b (CQW23_08898), PP2C (CQW23_15893), ABI5 (CQW23_29948), P450 (CQW23_16085), Aldedh1 (CQW23_06433), GDA (CQW23_12764), Aldedh2 (CQW23_14182), and Aldedh3 (CQW23_11481), were validated by qRT-PCR. This study provides valuable insights into the genetic mechanisms underlying salt stress tolerance in pepper. It offers potential targets for future breeding efforts to enhance salt stress resilience in this crop.
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页数:17
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