GL-V9 induce apoptosis of CML cells via MAPK signaling pathway

被引：0
|
作者：
Jiang, Fengyu [1 ]
Xue, Yangyang [1 ]
Zhang, Qin [1 ]
Ma, Tonghui [1 ]
Li, Yongming [1 ]
Yu, Xiaoxuan [1 ,2 ]
机构：
[1] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resourc, Sch Med, Nanjing 210023, Peoples R China
[2] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Peoples R China
来源：
HELIYON | 2024年 / 10卷 / 14期
基金：
中国国家自然科学基金;
关键词：
GL-V9; CML; Apoptosis; MAPK;
D O I：
10.1016/j.heliyon.2024.e34030
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
GL-V9, a derivative of wogonin, has shown potent antitumor effects in various cancers, yet its impact on chronic myeloid leukemia (CML) remains unexplored. In this study, we found that GLV9 significantly decreased the viability of CML cells. Annexin V/PI staining demonstrated that GL-V9 induced apoptosis in a concentration-dependent manner. The JC-1 assay indicated a significant reduction in mitochondrial membrane potential (Delta Psi m) in cells treated with GL-V9. Additionally, GL-V9 altered reactive oxygen species (ROS) levels in CML cells. Through transcriptomic sequencing and Western blot analysis, we further revealed that GL-V9 activated the MAPK pathway. These results suggest that GL-V9 is a promising therapeutic candidate for CML.
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收藏
页数：10
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