Characterization of a decellularized pericardium extracellular matrix hydrogel for regenerative medicine: insights on animal-to-animal variability

被引:1
作者
Di Francesco, Dalila [1 ,2 ]
Marcello, Elena [3 ,4 ]
Casarella, Simona [2 ]
Copes, Francesco [1 ]
Chevallier, Pascale [1 ]
Carmagnola, Irene [3 ,4 ]
Mantovani, Diego [1 ]
Boccafoschi, Francesca [2 ]
机构
[1] Laval Univ, Canada Res Chair Tier I Innovat Surg, Dept Min Met Mat Engn & Regenerat Med, Lab Biomat & Bioengn,CHU Quebec Res Ctr, Quebec City, PQ, Canada
[2] Univ Piemonte Orientale, Dept Hlth Sci, Novara, Italy
[3] Politecn Torino, Dept Mech & Aerosp Engn, Turin, Italy
[4] Politecn Torino, PoliTO BioMed Lab, Turin, Italy
来源
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2024年 / 12卷
关键词
decellularized extracellular matrix; hydrogel; bovine pericardium; characterization; regenerative medicine; animal-to-animal variability; BOVINE PERICARDIUM; MACROPHAGE PHENOTYPE; STERILIZATION; SCAFFOLD; IMMUNOMODULATION; RECONSTRUCTION; BIOSCAFFOLDS; MATRIKINES; GROWTH; BRAIN;
D O I
10.3389/fbioe.2024.1452965
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In the past years, the use of hydrogels derived from decellularized extracellular matrix (dECM) for regenerative medicine purposes has significantly increased. The intrinsic bioactive and immunomodulatory properties indicate these materials as promising candidates for therapeutical applications. However, to date, limitations such as animal-to-animal variability still hinder the clinical translation. Moreover, the choice of tissue source, decellularization and solubilization protocols leads to differences in dECM-derived hydrogels. In this context, detailed characterization of chemical, physical and biological properties of the hydrogels should be performed, with attention to how these properties can be affected by animal-to-animal variability. Herein, we report a detailed characterization of a hydrogel derived from the decellularized extracellular matrix of bovine pericardium (dBP). Protein content, rheological properties, injectability, surface microstructure, in vitro stability and cytocompatibility were evaluated, with particular attention to animal-to-animal variability. The gelation process showed to be thermoresponsive and the obtained dBP hydrogels are injectable, porous, stable up to 2 weeks in aqueous media, rapidly degrading in enzymatic environment and cytocompatible, able to maintain cell viability in human mesenchymal stromal cells. Results from proteomic analysis proved that dBP hydrogels are highly rich in composition, preserving bioactive proteoglycans and glycoproteins in addition to structural proteins such as collagen. With respect to the chemical composition, animal-to-animal variability was shown, but the biological properties were not affected, which remained consistent in different batches. Taken together these results show that dBP hydrogels are excellent candidates for regenerative medicine applications.
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页数:15
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