Combined effects of nitrogen limitation and overexpression of malic enzyme gene on lipid accumulation in Dunaliella parva

被引：2
作者：
Liang, Shi-Yu [1 ,2 ]
Li, Li-Hua [1 ,2 ]
Ling, Meng-Xiang [1 ,2 ]
Mo, Lei [1 ,2 ]
Huang, Li-Mei [1 ,2 ]
Ruan, Ling-Ru [1 ,2 ]
Shang, Chang-Hua [1 ,2 ]
机构：
[1] Guangxi Normal Univ, Key Lab Ecol Rare & Endangered Species & Environm, Minist Educ, Guilin 541006, Guangxi, Peoples R China
[2] Guangxi Normal Univ, Guangxi Key Lab Landscape Resources Conservat & Su, Guilin 541006, Guangxi, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2024年 / 410卷
关键词：
Transgenic microalga; Nitrogen stress; Biomass composition; NADPH; Lipid content; Starch content; EXPRESSION; DEPRIVATION; BIODIESEL; CHLAMYDOMONAS; BIOFUELS; CARBON; NADPH; PCR;
D O I：
10.1016/j.biortech.2024.131291
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Overexpression of Dunaliella parva (D. D. parva) ) malic enzyme (ME) gene ( DpME ) significantly increased DpME expression and ME enzyme activity in transgenic D. parva. . Nitrogen limitation had an inhibitory effect on protein content, and DpME overexpression could improve protein content. Nitrogen limitation increased carbohydrate content, and Dunaliella parva overexpressing malic enzyme gene under nitrogen limitation (DpME-N-)- ) group showed the lowest starch content among all groups. Dunaliella parva overexpressing malic enzyme gene under nitrogen sufficient condition (DpME) and DpME-N-- groups showed considerably high mRNA levels of DpME. ME activity was significantly enhanced by DpME overexpression, and nitrogen limitation caused a smaller increase. DpME overexpression and nitrogen limitation obviously enhanced lipid accumulation, and DpME overexpression had more obvious effect. Compared with control (wild type), lipid content (68.97%) obviously increased in DpME-N-- group. This study indicated that the combination of DpME overexpression and nitrogen limitation was favorable to the production of microalgae biodiesel.
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页数：9
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