Biogenic Synthesis of Papain Conjugated Copper Metallic Nanoparticles and its Antibacterial and Antifungal Activity

被引：0

作者：

Kotakonda, Muddukrishnaiah ^{[1
]}

Sajisha, V. S. ^{[2
]}

Aiswarya, G. ^{[2
]}

Pakkiyan, Safeela Nasrin ^{[1
]}

Alungal, Najamol A. ^{[1
]}

Kiliyankandi, K. Mayoora ^{[1
]}

Kareth, Divya Thekke ^{[1
]}

Parambil, Naheeda Ashraf Verali ^{[1
]}

Mohan, Saranya Sasi ^{[1
]}

Sheeba, Renjini Anil ^{[1
]}

Veettil, Sarika Puthiya ^{[1
]}

Joseph, Dhanish ^{[3
]}

Kakkattummal, Nishad ^{[1
]}

Haleem, M. P. Afsal Bin ^{[4
]}

Mayyeri, Safeera ^{[1
]}

Koyilott, Thasneem Chemban ^{[1
]}

Nalakath, Nasiya ^{[1
]}

Thavamani, B. Samuel ^{[5
]}

Rani, J. Famila ^{[6
]}

Periyasamy, Aruna ^{[7
]}

Rajesh, Chellappa, V ^{[8
]}

Shanmugam, Rameswari ^{[9
]}

Poornima, Marimuthu ^{[9
]}

Raju, Tina ^{[1
,10
]}

Roshni, E. R.

Panicke, Lekshmi M. S. ^{[11
]}

Saranya, K. G. ^{[12
]}

Kallungal, Sirajudheen Mukriyan

Shilpa, V. P. ^{[1
]}

机构：

[1] Jamia Salafiya Pharm Coll, Dept Pharm, Pulikkal, Kerala, India

[2] JDT Islam Coll Pharm, Dept Pharmaceut, Jamiath Hill, Calicut, Kerala, India

[3] Nirmala Coll Pharm, Dept Pharmaceut, Muvattupuzha, Kerala, India

[4] Sanjo Coll Pharmaceut Studies, Dept Pharm, Palakkad, Kerala, India

[5] Ahalia Sch Pharm, Dept Pharmacognosy, Ahalia Campus, Palakkad, Kerala, India

[6] JKKN Coll Pharm, Dept Pharmaceut Chem, Salem, Tamil Nadu, India

[7] Annamalai Univ, Dept Biochem & Biotechnol, Chidambaram, Tamil Nadu, India

[8] United Coll Pharm, Dept Pharmaceut, Coimbatore, Tamil Nadu, India

[9] Sree Sastha Pharm Coll, Dept Pharmaceut Chem, Chembarambakkam, Tamil Nadu, India

[10] DM WIMS Coll Pharm, Dept Pharmaceut, Wayanad, Kerala, India

[11] Mar Dioscorus Coll Pharm, Thiruvananthapuram 695017, Kerala, India

[12] Nirmala Coll Hlth Sci, Dept Pharmaceut, Chalakudy, Kerala, India

来源：

IRANIAN JOURNAL OF MATERIALS SCIENCE AND ENGINEERING | 2024年 / 21卷 / 03期

关键词：

Papain; Copper sulphate; Nanoparticles; SE-SEM; HRTEM; Antibacterial; Antifungal;

D O I：

10.22068/ijmse.3547

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Biogenic synthesis of papain-conjugated copper metallic Nanoparticles and their antibacterial and antifungal activities Papain metallic conjugated nanoparticles (Papain-CuNPs) were synthesised using Papain and CuSO4.5H(2)O. Papain-CuNPs were characterized using UV-visible spectroscopy, FT-IR, HR-TEM, XRD, FE-SEM, zeta potential, and a zeta sizer. The antibacterial activity of papain-CuNPs against human infectious microorganisms (Citrobacter spp, Pseudomonas aeruginosa and Candida albicans) was investigated. The mechanism of action of papain-CuNPs was evaluated using FE-SEM and HRTM. UV spectroscopy confirmed the plasma resonance (SPR) at 679 nm, which indicated the formation of papain-CuNPs. The FT-IR spectrum absorbance peaks at 3927, 3865, 3842, 3363, 2978, and 2900 cm(-1) indicate the presence of O-H and N-H of the secondary amine, and peaks at 1643 and 1572 cm(-1) represent C=O functional groups in Papain-CuNPs. EDAX analysis confirmed the presence of copper in the papain-CuNPs. The zeta potential (-42.6 mV) and zeta size (99.66 d. nm) confirmed the stability and size of the nanoparticles. XRD confirmed the crystalline nature of the papain-CuNPs. FE-SEM and HRTM showed an oval structure, and the nano particles' 16.71244-34.84793 nm. The synthesized papain-NPs showed significant antibacterial activity against clinical P. aeruginosa (15 mm). MIC 125 mu g/ml) showed bactericidal activity against P. aeruginosa and the mechanism of action of Papain-NPs was confirmed using an electron microscope by observing cell damage and cell shrinking. Papain-CuNPs have significant antibacterial activity and are thus used in the treatment of P. aeruginosa infections

引用

页码：1 / 10

页数：10

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