Nanosilicate-reinforced GelMA-PEGDA hydrogel promotes angiogenesis for bone regeneration

被引：3

作者：

Long, Jingjunjiao ^{[1
,2
]}

Luo, Yi ^{[1
,2
]}

Wang, Yitian ^{[1
,2
]}

Etxeberria, Alaitz Etxabide ^{[3
]}

Xing, Fei ^{[1
]}

Li, Zhuangzhuang ^{[1
,2
]}

Zhou, Yong ^{[1
,2
]}

Lu, Minxun ^{[1
,2
]}

Gong, Taojun ^{[1
,2
]}

Sun, Yong ^{[4
]}

Min, Li ^{[1
,2
]}

Fan, Yujiang ^{[4
]}

Tu, Chongqi ^{[1
,2
]}

Zhang, Xingdong ^{[4
]}

机构：

[1] Sichuan Univ, West China Hosp, Dept Orthoped Surg, 37 Guoxue Rd, Chengdu 610041, Sichuan, Peoples R China

[2] Model Worker & Craftsman Talent Innovat Workshop S, 37 Guoxue Rd, Chengdu 610041, Sichuan, Peoples R China

[3] Univ Basque Country UPV EHU, Escuela Ingn Gipuzkoa, BIOMAT Res Grp, Plaza Europa 1, Donostia San Sebastian 20018, Spain

[4] Sichuan Univ, Natl Engn Res Ctr Biomat, Chengdu 610064, Sichuan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 277卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Reinforced hydrogel; GelMA-PEGDA; Nanosilicate; Osteogenesis; Angiogenesis; GELATIN-METHACRYLOYL HYDROGEL; SCAFFOLDS;

D O I：

10.1016/j.ijbiomac.2024.133202

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bone tissue engineering has emerged as a pivotal field addressing the critical clinical needs of bone fractures. This study focused on developing multi-composite hydrogels by synergizing biocompatible GelMA macromolecules with synthetic PEGDA and reinforcing them with nanosilicates (SN). The incorporation of SN introduces crucial trace elements such as silicon, magnesium, and lithium, promoting both angiogenesis and osteogenesis. Characterizations revealed that PEGDA significantly reinforced the composite hydrogels' stability, while SN further enhanced the mechanical integrity of the GelMA-PEGDA-SN (GPS) hydrogels. Cell studies designated that GPS improved cell proliferation and migration, angiogenic VEGF/eNOS expression and osteogenic differentiation. In vivo experiments showed that GPS hydrogels effectively enhanced calvarial bone healing, with the GPS-2 formulation (2 % SN) displaying superior bone coverage and increased vascular formation. Assessments of osteogenic formation and the angiogenic marker CD31 validated the comprehensive bone regeneration potential of GPS hydrogels. These findings highlight the significant promise of GPS hydrogels in fostering bone healing with promoted angiogenesis.

引用

页数：11

共 48 条

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