Single molecule imaging unveils cellular architecture, dynamics and mechanobiology

被引：2

作者：

Chen, Tianchi ^{[1
]}

Iannone, Gregory G. ^{[1
]}

机构：

[1] Univ Bordeaux, Interdisciplinary Inst Neurosci, CNRS, UMR 5297, F-33000 Bordeaux, France

来源：

CURRENT OPINION IN CELL BIOLOGY | 2024年 / 88卷

关键词：

FOCAL ADHESION; NANOSCALE ARCHITECTURE; SUPERRESOLUTION MICROSCOPY; TENSION; MEMBRANE; CADHERIN; REVEALS; ACTIN; FORCE; ORGANIZATION;

D O I：

10.1016/j.ceb.2024.102369

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The biomechanical regulation of the cytoskeleton and cell adhesions underlies various essential cellular functions. Studying them requires visualizing their nanostructure and molecular dynamics with evermore precise spatio-temporal resolution. In this review we will focus on the recent advances in single molecule fluorescence imaging techniques and discuss how they improve our understanding of mechanically sensitive cellular structures such as adhesions and the cytoskeleton. We will also discuss future directions for research, emphasizing on the 3D nature of cellular structures and tissues, their mechanical regulation at the molecule level, as well as how super-resolution microscopy will enhance our knowledge on protein structure and conformational changes in the cellular context.

引用

页数：9

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