Secondary metabolites with antimicrobial activity produced by thermophilic bacteria from a high-altitude hydrothermal system

被引:1
作者
Pardo-Este, Coral [1 ]
Cortes, Johanna [2 ]
Castro-Severyn, Juan [3 ]
Perez, Vilma [4 ,5 ]
Henriquez-Aedo, Karem [6 ]
Cuadros, Fabian [2 ]
Yanez, Carolina [2 ]
Cuadros-Orellana, Sara [7 ]
Dorador, Cristina [8 ]
Molina, Veronica [9 ,10 ,11 ]
Eissler, Yoanna [12 ]
Paquis, Pablo [1 ]
Jeffrey, Wade H. [13 ]
Pozo, Patricia [14 ]
Perez, Pablo A. [14 ]
Hengst, Martha B. [1 ]
机构
[1] Univ Catolica Norte, Fac Ciencias, Dept Ciencias Farmaceut, Lab Ecol Mol & Microbiol Aplicada, Antofagasta, Chile
[2] Pontificia Univ Catolica Valparaiso, Microbial Ecol Rhizosphere Grp, Valparaiso, Chile
[3] Univ Catolica Norte, Dept Ingn Quim, Lab Microbiol Aplicada & Extremofilos, Antofagasta, Chile
[4] Univ Adelaide, Australian Ctr Ancient DNA ACAD, Adelaide, SA, Australia
[5] Univ Adelaide, Ctr Excellence Australian Biodivers & Heritage, Adelaide, SA, Australia
[6] Univ Bio Bio, Fac Ciencias, Dept Ciencias Bas, Lab Biotecnol & Genet Alimentos, Chillan, Chile
[7] Univ Catolica Maule, Fac Ciencias Agr & Forestales, Ctr Biotecnol Recursos Nat, Lab Genom, Talca, Chile
[8] Univ Antofagasta, Fac Ciencias Mar & Recursos Biol, Dept Biotecnol, Antofagasta, Chile
[9] Univ Playa Ancha, Fac Ciencias Nat & Exactas, Dept Ciencias & Geog, Valparaiso, Chile
[10] Univ Playa Ancha, HUB Ambiental UPLA, Valparaiso, Chile
[11] Univ Concepcion, Ctr COPAS Coastal, Concepcion, Chile
[12] Univ Valparaiso, Ctr Neurobiol & Fisiopatol Integrat, Lab Virol, Inst Quim & Bioquim,Fac Ciencias, Valparaiso, Chile
[13] Univ West Florida, Ctr Environm Diagnost & Bioremediat, Pensacola, FL USA
[14] Univ Catolica Norte, Fac Ciencias, Dept Ciencias Farmaceut, Antofagasta, Chile
关键词
bioprospection; secondary metabolites; thermophilic bacteria; antimicrobial activity; bioactive compounds; STIGMATELLA-AURANTIACA; BIOSYNTHESIS; ANTIBIOTICS; THIAZOLE; 10-DEOXYMETHYNOLIDE; ANNOTATION; METHYMYCIN; DIVERSITY; SCAFFOLDS; DISCOVERY;
D O I
10.3389/fmicb.2024.1477458
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Thermophilic microorganisms possess several adaptations to thrive in high temperature, which is reflected as biosynthesis of proteins and thermostable molecules, isolation and culture represent a great methodological challenge, therefore High throughput sequencing enables screening of the whole bacterial genome for functional potential, providing rapid and cost-effective information to guide targeted cultures for the identification and characterization of novel natural products. In this study, we isolated two thermophilic bacterial strains corresponding to Bacillus LB7 and Streptomyces LB8, from the microbial mats in the Atacama Desert. By combining genome mining, targeted cultures and biochemical characterization, we aimed to identify their capacity to synthesize bioactive compounds with antimicrobial properties. Additionally, we determined the capability to produce bioactive compounds under controlled in vitro assays and detected by determining their masses by Thin-Layer Chromatography/Mass Spectrometry (TLC/MS). Overall, both isolates can produce antimicrobial (e.g., Myxalamide C by-product) and antioxidants (e.g. Dihydroxymandelic Acid, Amide biotine and Flavone by-products) compounds. Bacillus LB7 strain possesses a more diverse repertoire with 51.95% of total metabolites unmatched, while Streptomyces LB8 favors mainly antioxidants, but has over 70% of unclassified compounds, highlighting the necessity to study and elucidate the structure of novel compounds. Based on these results, we postulate that the uncultured or rare cultured thermophiles inhabiting high-altitude hydrothermal ecosystems in the Atacama Desert offer a promising opportunity to the study of novel microbial bioactive compounds.
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页数:13
相关论文
共 86 条
[1]   Inhibition of bacterial activities by solar radiation in nearshore waters and the Gulf of Mexico [J].
Aas, P ;
Lyons, MM ;
Pledger, R ;
Mitchell, DL ;
Jeffrey, WH .
AQUATIC MICROBIAL ECOLOGY, 1996, 11 (03) :229-238
[2]   Large Differences in Bacterial Community Composition among Three Nearby Extreme Waterbodies of the High Andean Plateau [J].
Aguilar, Pablo ;
Scosta, Eduardo ;
Dorador, Cristina ;
Sommaruga, Ruben .
FRONTIERS IN MICROBIOLOGY, 2016, 7
[3]   Chemoenzymatic synthesis of the polyketide macrolactone 10-deoxymethynolide [J].
Aldrich, CC ;
Venkatraman, L ;
Sherman, DH ;
Fecik, RA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (25) :8910-8911
[4]  
Andrews S, 2010, "FastQC: A Quality Control Tool for High Throughput Sequence Data."
[5]   New method for caffeine quantification by planar chromatography coupled with electropray ionization mass spectrometry using stable isotope dilution analysis [J].
Aranda, Mario ;
Morlock, Gertrud .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007, 21 (07) :1297-1303
[6]   SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing [J].
Bankevich, Anton ;
Nurk, Sergey ;
Antipov, Dmitry ;
Gurevich, Alexey A. ;
Dvorkin, Mikhail ;
Kulikov, Alexander S. ;
Lesin, Valery M. ;
Nikolenko, Sergey I. ;
Son Pham ;
Prjibelski, Andrey D. ;
Pyshkin, Alexey V. ;
Sirotkin, Alexander V. ;
Vyahhi, Nikolay ;
Tesler, Glenn ;
Alekseyev, Max A. ;
Pevzner, Pavel A. .
JOURNAL OF COMPUTATIONAL BIOLOGY, 2012, 19 (05) :455-477
[7]   Study of marine microorganism metabolites: new resources for bioactive natural products [J].
Barzkar, Noora ;
Sukhikh, Stanislav ;
Babich, Olga .
FRONTIERS IN MICROBIOLOGY, 2024, 14
[8]  
Benson DA, 2013, NUCLEIC ACIDS RES, V41, pD36, DOI [10.1093/nar/gkr1202, 10.1093/nar/gks1195, 10.1093/nar/gkn723, 10.1093/nar/gkp1024, 10.1093/nar/gkg057, 10.1093/nar/gkw1070, 10.1093/nar/gkl986, 10.1093/nar/gkx1094, 10.1093/nar/gkq1079]
[9]  
Berridge MV, 2005, BIOTECHNOL ANN REV, V11, P127, DOI 10.1016/S1387-2656(05)11004-7
[10]   Responses of Microorganisms to Osmotic Stress [J].
Bremer, Erhard ;
Kraemer, Reinhard .
ANNUAL REVIEW OF MICROBIOLOGY, VOL 73, 2019, 73 :313-+