Duck enteritis virus LORF4 gene is a late gene and nonessential for virus replication in vitro

被引：0
作者：
Huang, Jie [1 ,2 ,3 ,4 ,5 ]
Wang, Mingshu [1 ,2 ,3 ,4 ,5 ]
Cheng, Anchun [1 ,2 ,3 ,4 ,5 ]
Yang, Qiao [1 ,2 ,3 ,4 ,5 ]
Tian, Bin [1 ,2 ,3 ,4 ,5 ]
Wu, Ying [1 ,2 ,3 ,4 ,5 ]
Ou, Xumin [1 ,2 ,3 ,4 ,5 ]
Sun, Di [1 ,2 ,3 ,4 ,5 ]
He, Yu [1 ,2 ,3 ,4 ,5 ]
Wu, Zhen [1 ,2 ,3 ,4 ,5 ]
Zhao, Xinxin [1 ,2 ,3 ,4 ,5 ]
Zhang, Shaqiu [1 ,2 ,3 ,4 ,5 ]
Huang, Juan [1 ,2 ,3 ,4 ,5 ]
Zhu, Dekang [1 ,2 ,3 ,4 ,5 ]
Jia, Renyong [1 ,2 ,3 ,4 ,5 ]
Liu, Mafeng [1 ,2 ,3 ,4 ,5 ]
Chen, Shun [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Minist Educ Peoples Republ China, Engn Res Ctr Southwest Anim Dis Prevent & Control, Chengdu 611130, Peoples R China
[2] Sichuan Agr Univ, Coll Vet Med, Key Lab Anim Dis & Human Hlth Sichuan Prov, Chengdu 611130, Peoples R China
[3] Sichuan Agr Univ, Int Joint Res Ctr Anim Dis Prevent & Control Sichu, Chengdu 611130, Peoples R China
[4] Sichuan Agr Univ, Inst Vet Med & Immunol, Coll Vet Med, Chengdu 611130, Peoples R China
[5] Sichuan Agr Univ, Coll Vet Med, Res Ctr Avian Dis, Chengdu 611130, Peoples R China
来源：
POULTRY SCIENCE | 2024年 / 103卷 / 12期
关键词：
Duck enteritis virus; LORF4; specific gene; virus replication; nonessential gene; COMPLETE GENOMIC SEQUENCE; UNIQUE; PLAGUE; PLAYS;
D O I：
10.1016/j.psj.2024.104275
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Duck enteritis virus (DEV) is an avian alpha-herpesvirus that primarily causes an acute and highly contagious infectious disease of ducks. The LORF4 gene is one of the specific genes of DEV, with limited reports on its biological characteristics and functions. This study investigates the basic biological properties of LORF4 protein (pLORF4). The results show that DEV LORF4 is a late gene mainly localized in the cytoplasm of DEV-infected DEF. To explore the role of pLORF4 in the DEV replication life cycle, a recombinant virus lacking pLORF4 expression was constructed. The results showed that pLORF4 is not essential for virus replication and does not affect virus adsorption, assembly and release, it plays a positive role in virus invasion and DNA replication. In summary, this study provides a foundation for further research on the function of the LORF4 gene.
引用
收藏
页数：11
相关论文
共 25 条
  • [1] THE MAREKS-DISEASE VIRUS (MDV) UNIQUE SHORT REGION - ALPHAHERPESVIRUS-HOMOLOGOUS, FOWLPOX VIRUS-HOMOLOGOUS, AND MDV-SPECIFIC GENES
    BRUNOVSKIS, P
    VELICER, LF
    [J]. VIROLOGY, 1995, 206 (01) : 324 - 338
  • [2] STRUCTURAL-ANALYSIS AND TRANSCRIPTIONAL MAPPING OF THE MAREKS-DISEASE VIRUS GENE ENCODING PP38, AN ANTIGEN ASSOCIATED WITH TRANSFORMED-CELLS
    CUI, ZZ
    LEE, LF
    LIU, JL
    KUNG, HJ
    [J]. JOURNAL OF VIROLOGY, 1991, 65 (12) : 6509 - 6515
  • [3] Duck virus enteritis (duck plague) - a comprehensive update
    Dhama, Kuldeep
    Kumar, Naveen
    Saminathan, Mani
    Tiwari, Ruchi
    Karthik, Kumaragurubaran
    Kumar, M. Asok
    Palanivelu, M.
    Shabbir, Muhammad Zubair
    Malik, Yashpal Singh
    Singh, Raj Kumar
    [J]. VETERINARY QUARTERLY, 2017, 37 (01) : 57 - 80
  • [4] Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1
    Guo, Yufei
    Cheng, Anchun
    Wang, Mingshu
    Shen, Chanjuan
    Jia, Renyong
    Chen, Shun
    Zhang, Na
    [J]. VIROLOGY JOURNAL, 2009, 6
  • [5] Herpes Simplex Virus 1 pUL34 Plays a Critical Role in Cell-to-Cell Spread of Virus in Addition to Its Role in Virus Replication
    Haugo, Alison C.
    Szpara, Moriah L.
    Parsons, Lance
    Enquist, Lynn W.
    Roller, Richard J.
    [J]. JOURNAL OF VIROLOGY, 2011, 85 (14) : 7203 - 7215
  • [6] Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1
    He, Tianqiong
    Wang, Mingshu
    Cheng, Anchun
    Yang, Qiao
    Jia, Renyong
    Wu, Ying
    Huang, Juan
    Chen, Shun
    Zhao, Xin-Xin
    Liu, Mafeng
    Zhu, Dekang
    Zhang, Shaqiu
    Ou, Xuming
    Mao, Sai
    Gao, Qun
    Sun, Di
    Wen, XinJian
    Tian, Bin
    Liu, Yunya
    Yu, Yanling
    Zhang, Ling
    Pan, Leichang
    Chen, Xiaoyue
    [J]. VETERINARY RESEARCH, 2020, 51 (01)
  • [7] Hu Y., 2020, Construction and immune effect evaluation of duck plague virus LORF4-deletion strain
  • [8] The complete unique long sequence and the overall genomic organization of the GA strain of Marek's disease virus
    Lee, LF
    Wu, P
    Sui, DX
    Ren, DL
    Kamil, J
    Kung, HJ
    Witter, RL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (11) : 6091 - 6096
  • [9] Latest Insights into Unique Open Reading Frames Encoded by Unique Long (UL) and Short (US) Regions of Marek's Disease Virus
    Liao, Yifei
    Lupiani, Blanca
    Reddy, Sanjay M.
    [J]. VIRUSES-BASEL, 2021, 13 (06):
  • [10] Deletion of LORF9 but not LORF10 attenuates Marek 's disease virus pathogenesis
    Liao, Yifei
    Sun, Aijun
    Zhuang, Guoqing
    Lupiani, Blanca
    Reddy, Sanjay M.
    [J]. VETERINARY MICROBIOLOGY, 2020, 251
123