FilamentID reveals the composition and function of metabolic enzyme polymers during gametogenesis

被引：8

作者：

Hugener, Jannik ^{[1
,2
,3
]}

Xu, Jingwei ^{[1
]}

Wettstein, Rahel ^{[2
,3
]}

Ioannidi, Lydia ^{[3
]}

Velikov, Daniel ^{[3
,4
,5
]}

Wollweber, Florian ^{[1
]}

Henggeler, Adrian ^{[2
,3
]}

Matos, Joao ^{[2
,3
]}

Pilhofer, Martin ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Inst Mol Biol & Biophys, CH-8093 Zurich, Switzerland

[2] Swiss Fed Inst Technol, Inst Biochem, CH-8093 Zurich, Switzerland

[3] Univ Vienna, Max Perutz Labs, A-1030 Vienna, Austria

[4] Univ Vienna, Doctoral Sch, Vienna Bioctr PhD Program, A-1030 Vienna, Austria

[5] Med Univ Vienna, A-1030 Vienna, Austria

来源：

CELL | 2024年 / 187卷 / 13期

基金：

欧洲研究理事会;

关键词：

ACETYL-COA SYNTHETASE; COENZYME-A SYNTHETASE; CRYO-EM; SACCHAROMYCES-CEREVISIAE; HISTONE ACETYLATION; REGULATORY CONTROL; CRYSTAL-STRUCTURE; MEIOSIS; RESOLUTION; GENE;

D O I：

10.1016/j.cell.2024.04.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gamete formation and subsequent offspring development often involve extended phases of suspended cellular development or even dormancy. How cells adapt to recover and resume growth remains poorly understood. Here, we visualized budding yeast cells undergoing meiosis by cryo-electron tomography (cryoET) and discovered elaborate filamentous assemblies decorating the nucleus, cytoplasm, and mitochondria. To determine filament composition, we developed a "filament identification"(FilamentID) workflow that combines multiscale cryoET/cryo-electron microscopy (cryoEM) analyses of partially lysed cells or organelles. FilamentID identified the mitochondrial filaments as being composed of the conserved aldehyde dehydrogenase Ald4 ALDH2 and the nucleoplasmic/cytoplasmic filaments as consisting of acetyl-coenzyme A (CoA) synthetase Acs1ACSS2. ACSS2 . Structural characterization further revealed the mechanism underlying polymerization and enabled us to genetically perturb filament formation. Acs1 polymerization facilitates the recovery of chronologically aged spores and, more generally, the cell cycle re-entry of starved cells. FilamentID is broadly applicable to characterize filaments of unknown identity in diverse cellular contexts.

引用

页码：3303 / 3318.e18

页数：35

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