Multiwavelength Photoacoustic Doppler Flowmetry of Living Microalgae Cells

被引:0
|
作者
Farooq, Tayyab [1 ,2 ]
Wu, Xiuru [1 ]
Yan, Sheng [3 ]
Fang, Hui [1 ]
机构
[1] Inst Microscale Optoelect, Nanophoton Res Ctr, Shenzhen Key Lab Microscale Opt Informat Technol, Shenzhen 518060, Peoples R China
[2] Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen 518060, Peoples R China
[3] Shenzhen Univ, Inst Adv Study, Shenzhen 518060, Peoples R China
来源
BIOSENSORS-BASEL | 2024年 / 14卷 / 08期
基金
中国国家自然科学基金;
关键词
photoacoustic Doppler (PAD); Doppler flowmetry; supercontinuum laser; microalgae absorption spectroscopy; microalgae flow measurement; FLOW-CYTOMETRY; SPECTROSCOPY; CHLOROPHYLL; FUCOXANTHIN; STATE;
D O I
10.3390/bios14080397
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Photoacoustics can provide a direct measurement of light absorption by microalgae depending on the photosynthesis pigment within them. In this study, we have performed photoacoustic flowmetry on living microalgae cells to measure their flow characteristics, which include flow speed, flow angle, flow direction, and, more importantly, the photoacoustic absorption spectrum, all by observing the photoacoustic Doppler power spectra during their flowing state. A supercontinuum pulsed laser with a high repetition frequency is used as the light source: through intensity modulation at a specified frequency, it can provide wavelength-selectable excitation of a photoacoustic signal centered around this frequency. Our approach can be useful to simultaneously measure the flow characteristics of microalgae and easily discriminate their different species with high accuracy in both static and dynamic states, thus facilitating the study of their cultivation and their role in our ecosystem.
引用
收藏
页数:16
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