Standardization and quality assessment for human intestinal organoids

被引：4

作者：

Lee, Hana ^{[1
,2
]}

Yang, Seunghye ^{[2
,3
]}

Lee, Kyung Jin ^{[2
,3
]}

Kim, Si-Na ^{[3
]}

Jeong, Ji-Seon ^{[2
,4
]}

Kim, Ki Young ^{[2
,5
]}

Jung, Cho-Rok ^{[1
,2
,6
]}

Jeon, Sooyeon ^{[7
]}

Kwon, Dayeon ^{[7
]}

Lee, Sungin ^{[7
]}

Lee, Hanbyeol ^{[7
]}

Park, Chihye ^{[7
]}

Ahn, Sun-Ju ^{[2
,7
]}

Yoo, Jongman ^{[2
,3
,8
]}

Son, Mi-Young ^{[1
,2
,6
,9
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol KRIBB, Daejeon, South Korea

[2] Sungkyunkwan Univ, Inst Quantum Biophys, Dept Biophys, Organoid Stand Initiat OSI, Suwon, South Korea

[3] ORGANOIDSCI, Seongnam, South Korea

[4] Korea Res Inst Stand & Sci KRISS, Daejeon, South Korea

[5] Korea Res Inst Chem Technol, Therapeut & Biotechnol Div, Daejeon, South Korea

[6] Korea Univ Sci & Technol UST, KRIBB Sch Biosci, Daejeon, South Korea

[7] Sungkyunkwan Univ, Dept Biophys, Digital Hlth Lab, Suwon, South Korea

[8] CHA Univ, Sch Med, Dept Microbiol, Seongnam Si, South Korea

[9] Sungkyunkwan Univ, Dept Biol Sci, Suwon, South Korea

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2024年 / 12卷

关键词：

standardization; guideline; human adult stem cell (hASC); human pluripotent stem cell (hPSC); human intestinal organoid (hIO); STEM-CELLS; IN-VITRO; SELF-RENEWAL; DIFFERENTIATION; COLON; CRYPT; REGENERATION;

D O I：

10.3389/fcell.2024.1383893

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To enhance the practical application of intestinal organoids, it is imperative to establish standardized guidelines. This proposed standardization outlines a comprehensive framework to ensure consistency and reliability in the development, characterization, and application of intestinal organoids. The recommended guidelines encompass crucial parameters, including culture conditions, critical quality attributes, quality control measures, and functional assessments, aimed at fostering a standardized approach across diverse research initiatives. The implementation of these guidelines is anticipated to significantly contribute to the reproducibility and comparability of results in the burgeoning field of intestinal organoid research.

引用

页数：16

共 50 条

[41] Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids [J].

Hill, David R. ;

Huang, Sha ;

Tsai, Yu-Hwai ;

Spence, Jason R. ;

Young, Vincent B. .

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2017, (130)

[42] Generation of Differentiating and Long-Living Intestinal Organoids Reflecting the Cellular Diversity of Canine Intestine [J].

Kramer, Nina ;

Pratscher, Barbara ;

Meneses, Andre M. C. ;

Tschulenk, Waltraud ;

Walter, Ingrid ;

Swoboda, Alexander ;

Kruitwagen, Hedwig S. ;

Schneeberger, Kerstin ;

Penning, Louis C. ;

Spee, Bart ;

Kieslinger, Matthias ;

Brandt, Sabine ;

Burgener, Iwan A. .

CELLS, 2020, 9 (04)

[43] Drivers of transcriptional variance in human intestinal epithelial organoids [J].

Criss, Zachary K. ;

Bhasin, Nobel ;

Di Rienzi, Sara C. ;

Rajan, Anubama ;

Deans-Fielder, Kali ;

Swaminathan, Ganesh ;

Kamyabi, Nabiollah ;

Zeng, Xi-Lei ;

Doddapaneni, Harsha ;

Menon, Vipin K. ;

Chakravarti, Deepavali ;

Estrella, Clarissa ;

Yu, Xiaomin ;

Patil, Ketki ;

Petrosino, Joseph F. ;

Fleet, James C. ;

Verzi, Michael P. ;

Christakos, Sylvia ;

Helmrath, Michael A. ;

Arimura, Sumimasa ;

DePinho, Ronald A. ;

Britton, Robert A. ;

Maresso, Anthony W. ;

Grande-Allen, K. Jane ;

Blutt, Sarah E. ;

Crawford, Sue E. ;

Estes, Mary K. ;

Ramani, Sasirekha ;

Shroyer, Noah F. .

PHYSIOLOGICAL GENOMICS, 2021, 53 (11) :486-508

[44] In-silico and in-vitro morphometric analysis of intestinal organoids [J].

Montes-Olivas, Sandra ;

Legge, Danny ;

Lund, Abbie ;

Fletcher, Alexander G. ;

Williams, Ann C. ;

Marucci, Lucia ;

Homer, Martin .

PLOS COMPUTATIONAL BIOLOGY, 2023, 19 (08)

[45] Establishment of human intestinal organoids derived from commercially available cryopreserved intestinal epithelium and evaluation for pharmacokinetic study [J].

Okada, Kentaro ;

Yokota, Jumpei ;

Yamashita, Tomoki ;

Inui, Tatsuya ;

Kishimoto, Wataru ;

Nakase, Hiroshi ;

Mizuguchi, Hiroyuki .

DRUG METABOLISM AND PHARMACOKINETICS, 2024, 54

[46] Generation and Manipulation of Rat Intestinal Organoids [J].

Zagoren, Eleanor ;

Santos, Anderson K. ;

Ameen, Nadia A. ;

Sumigray, Kaelyn .

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2023, (196)

[47] CDX2-induced intestinal metaplasia in human gastric organoids derived from induced pluripotent stem cells [J].

Koide, Takahiro ;

Koyanagi-Aoi, Michiyo ;

Uehara, Keiichiro ;

Kakeji, Yoshihiro ;

Aoi, Takashi .

ISCIENCE, 2022, 25 (05)

[48] Generation of intestinal organoids derived from human pluripotent stem cells for drug testing [J].

Yoshida, Shinpei ;

Miwa, Hiroto ;

Kawachi, Tomoyuki ;

Kume, Shoen ;

Takahashi, Koji .

SCIENTIFIC REPORTS, 2020, 10 (01)

[49] Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells [J].

Mithal, Aditya ;

Capilla, Amalia ;

Heinze, Dar ;

Berical, Andrew ;

Villacorta-Martin, Carlos ;

Vedaie, Marall ;

Jacob, Anjali ;

Abo, Kristine ;

Szymaniak, Aleksander ;

Peasley, Megan ;

Stuffer, Alexander ;

Mahoney, John ;

Kotton, Darrell N. ;

Hawkins, Finn ;

Mostoslavsky, Gustavo .

NATURE COMMUNICATIONS, 2020, 11 (01)

[50] Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids [J].

Martinez-Silgado, Adriana ;

Yengej, Fjodor A. Yousef ;

Puschhof, Jens ;

Geurts, Veerle ;

Boot, Charelle ;

Geurts, Maarten H. ;

Rookmaaker, Maarten B. ;

Verhaar, Marianne C. ;

Beumer, Joep ;

Clevers, Hans .

STAR PROTOCOLS, 2022, 3 (03)

← 1 2 3 4 5 →