Standardization and quality assessment for human intestinal organoids

被引:1
|
作者
Lee, Hana [1 ,2 ]
Yang, Seunghye [2 ,3 ]
Lee, Kyung Jin [2 ,3 ]
Kim, Si-Na [3 ]
Jeong, Ji-Seon [2 ,4 ]
Kim, Ki Young [2 ,5 ]
Jung, Cho-Rok [1 ,2 ,6 ]
Jeon, Sooyeon [7 ]
Kwon, Dayeon [7 ]
Lee, Sungin [7 ]
Lee, Hanbyeol [7 ]
Park, Chihye [7 ]
Ahn, Sun-Ju [2 ,7 ]
Yoo, Jongman [2 ,3 ,8 ]
Son, Mi-Young [1 ,2 ,6 ,9 ]
机构
[1] Korea Res Inst Biosci & Biotechnol KRIBB, Daejeon, South Korea
[2] Sungkyunkwan Univ, Inst Quantum Biophys, Dept Biophys, Organoid Stand Initiat OSI, Suwon, South Korea
[3] ORGANOIDSCI, Seongnam, South Korea
[4] Korea Res Inst Stand & Sci KRISS, Daejeon, South Korea
[5] Korea Res Inst Chem Technol, Therapeut & Biotechnol Div, Daejeon, South Korea
[6] Korea Univ Sci & Technol UST, KRIBB Sch Biosci, Daejeon, South Korea
[7] Sungkyunkwan Univ, Dept Biophys, Digital Hlth Lab, Suwon, South Korea
[8] CHA Univ, Sch Med, Dept Microbiol, Seongnam Si, South Korea
[9] Sungkyunkwan Univ, Dept Biol Sci, Suwon, South Korea
来源
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2024年 / 12卷
关键词
standardization; guideline; human adult stem cell (hASC); human pluripotent stem cell (hPSC); human intestinal organoid (hIO); STEM-CELLS; IN-VITRO; SELF-RENEWAL; DIFFERENTIATION; COLON; CRYPT; REGENERATION;
D O I
10.3389/fcell.2024.1383893
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To enhance the practical application of intestinal organoids, it is imperative to establish standardized guidelines. This proposed standardization outlines a comprehensive framework to ensure consistency and reliability in the development, characterization, and application of intestinal organoids. The recommended guidelines encompass crucial parameters, including culture conditions, critical quality attributes, quality control measures, and functional assessments, aimed at fostering a standardized approach across diverse research initiatives. The implementation of these guidelines is anticipated to significantly contribute to the reproducibility and comparability of results in the burgeoning field of intestinal organoid research.
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页数:16
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