Coating seeds with biocontrol bacteria-loaded sodium alginate/pectin hydrogel enhances the survival of bacteria and control efficacy against soil-borne vegetable diseases

被引:2
作者
Abdukerim, Rizwangul [1 ]
Li, Lei [1 ]
Li, Jun-Hui [1 ]
Xiang, Sheng [1 ]
Shi, Yan-Xia [1 ]
Xie, Xue-Wen [1 ]
Chai, A. -Li [1 ]
Fan, Teng-Fei [1 ]
Li, Bao-Ju [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China
基金
中国国家自然科学基金;
关键词
Sodium alginate; Pectin; Seed coating; Biocontrol; Plant disease; LACTOBACILLUS-ACIDOPHILUS; MECHANICAL-PROPERTIES; PECTIN; CALCIUM; GELATION; BEHAVIOR; QUALITY; GROWTH; GELS; MICROENCAPSULATION;
D O I
10.1016/j.ijbiomac.2024.135317
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microbial seed coatings serve as effective, labor-saving, and ecofriendly means of controlling soil-borne plant diseases. However, the survival of microbial agents on seed surfaces and in the rhizosphere remains a crucial challenge. In this work, we embedded a biocontrol bacteria (Bacillus subtilis ZF71) in sodium alginate (SA)/pectin (PC) hydrogel as a seed coating agent to control Fusarium root rot in cucumber. The formula of SA/PC hydrogel was optimized with the highest coating uniformity of 90 % in cucumber seeds. SA/PC hydrogel was characterized using rheological, gel content, and water content tests, thermal gravimetric analysis, and Fourier transform infrared spectroscopy. Bacillus subtilis ZF71 within the SA/PC hydrogel network formed a biofilm-like structure with a high viable cell content (8.30 log CFU/seed). After 37 days of storage, there was still a high number of Bacillus subtilis ZF71 cells (7.23 log CFU/seed) surviving on the surface of cucumber seeds. Pot experiments revealed a higher control efficiency against Fusarium root rot in ZF71-SA/PC cucumber seeds (53.26 %) compared with roots irrigated with a ZF71 suspension. Overall, this study introduced a promising microbial seed coating strategy based on biofilm formation that improved performance against soil-borne plant diseases.
引用
收藏
页数:12
相关论文
共 80 条
  • [11] Enhancement of adsorption ability of calcium alginate gel beads towards Pd(II) ion. A kinetic and equilibrium study on hybrid Laponite and Montmorillonite-alginate gel beads
    Cataldo, Salvatore
    Muratore, Nicola
    Orecchio, Santino
    Pettignano, Alberto
    [J]. APPLIED CLAY SCIENCE, 2015, 118 : 162 - 170
  • [12] Cerná M, 2003, CARBOHYD POLYM, V51, P383, DOI 10.1016/S0144-8617(02)00259-X
  • [13] Water resistance and mechanical properties of low methoxy-pectin nanocomposite film responses to interactions of Ca2+ ions and glycerol concentrations as crosslinking agents
    Chaichi, Maryam
    Badii, Fojan
    Mohammadi, Abdorreza
    Hashemi, Maryam
    [J]. FOOD CHEMISTRY, 2019, 293 : 429 - 437
  • [14] Swelling behavior and the release of protein from chitosan-pectin composite particles
    Chang, KLB
    Lin, J
    [J]. CARBOHYDRATE POLYMERS, 2000, 43 (02) : 163 - 169
  • [15] Enhance the resistance of probiotics by microencapsulation and biofilm construction based on rhamnogalacturonan I rich pectin
    Chen, Pin
    Tian, Jinhu
    Ren, Yanming
    Cheng, Huan
    Pan, Haibo
    Chen, Shiguo
    Ye, Xingqian
    Chen, Jianle
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2024, 258
  • [16] The Fusarium solani species complex: ubiquitous pathogens of agricultural importance
    Coleman, Jeffrey J.
    [J]. MOLECULAR PLANT PATHOLOGY, 2016, 17 (02) : 146 - 158
  • [17] Colla G, 2015, INT J PLANT PROD, V9, P171
  • [18] Investigation of pectin/starch hydrogel as a carrier for oral delivery of probiotic bacteria
    Dafe, Alireza
    Etemadi, Hossein
    Dilmaghani, Azita
    Mandavinia, Gholam Reza
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 97 : 536 - 543
  • [19] Desiccation tolerance of rhizobia when protected by synthetic polymers
    Deaker, Rosalind
    Roughley, Rodney J.
    Kennedy, Ivan R.
    [J]. SOIL BIOLOGY & BIOCHEMISTRY, 2007, 39 (02) : 573 - 580
  • [20] Sodium alginate potentiates antioxidant defense and PR proteins against early blight disease caused by Alternaria solani in Solanum lycopersicum Linn
    Dey, Priya
    Ramanujam, Ramani
    Venkatesan, Ganesan
    Nagarathnam, Radhakrishnan
    [J]. PLOS ONE, 2019, 14 (09):