Unraveling the role of the nucleocapsid protein in SARS-CoV-2 pathogenesis: From viral life cycle to vaccine development

被引：8

作者：

El-Maradny, Yousra A. ^{[1
,2
]}

Badawy, Moustafa A. ^{[3
]}

Mohamed, Kareem I. ^{[2
]}

Ragab, Renad F. ^{[2
]}

Moharm, Hamssa M. ^{[4
]}

Abdallah, Nada A. ^{[5
]}

Elgammal, Esraa M. ^{[2
]}

Rubio-Casillas, Alberto ^{[6
,7
]}

Uversky, Vladimir N. ^{[8
,9
]}

Redwan, Elrashdy M. ^{[10
,11
,12
]}

机构：

[1] City Sci Res & Technol Applicat SRTA City, Pharmaceut & Fermentat Ind Dev Ctr PFIDC, Alexandria 21934, Egypt

[2] Arab Acad Sci Technol & Maritime Transport AASTMT, Fac Pharm, Microbiol & Immunol, El Alamein 51718, Egypt

[3] Alexandria Univ, Fac Sci, Ind Microbiol & Appl Chem program, Alexandria, Egypt

[4] Alexandria Univ, Fac Agr, Biotechnol Dept, Genet, Alexandria, Egypt

[5] Alexandria Univ, Fac Agr, Med Plants Dept, Alexandria, Egypt

[6] Autlan Reg Hosp, Hlth Secretariat, Autlan de Navarro 48900, JAL, Mexico

[7] Univ Guadalajara, Autlan Reg Preparatory Sch, Biol Lab, Autlan de Navarro 48900, JAL, Mexico

[8] Univ S Florida, Morsani Coll Med, Dept Mol Med, Tampa, FL 33612 USA

[9] Univ S Florida, USF Hlth Byrd Alzheimers Res Inst, Morsani Coll Med, Tampa, FL 33612 USA

[10] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah, Saudi Arabia

[11] King Abdulaziz Univ, Ctr Excellence Bionanosci Res, Jeddah 21589, Saudi Arabia

[12] City Sci Res & Technol Applicat SRTA City, Genet Engn & Biotechnol Res Inst, Prot Res Dept, Therapeut & Protect Prot Lab, Alexandria 21934, Egypt

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 279卷

关键词：

Nucleocapsid; Extracellular vesicles; Ubiquitination; Liquid-liquid phase separation; SUMOylation; INTRINSICALLY UNSTRUCTURED PROTEINS; SARS CORONAVIRUS; DIMERIZATION DOMAIN; INTRACELLULAR DNA; STRESS GRANULES; TERMINAL DOMAIN; RICH MOTIF; DISORDER; RNA; SPIKE;

D O I：

10.1016/j.ijbiomac.2024.135201

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: The nucleocapsid protein (N protein) is the most abundant protein in SARS-CoV-2. Viral RNA and this protein are bound by electrostatic forces, forming cytoplasmic helical structures known as nucleocapsids. Subsequently, these nucleocapsids interact with the membrane (M) protein, facilitating virus budding into early secretory compartments. Scope of review: Exploring the role of the N protein in the SARS-CoV-2 life cycle, pathogenesis, post-sequelae consequences, and interaction with host immunity has enhanced our understanding of its function and potential strategies for preventing SARS-CoV-2 infection. Major conclusion: This review provides an overview of the N protein's involvement in SARS-CoV-2 infectivity, highlighting its crucial role in the virus-host protein interaction and immune system modulation, which in turn influences viral spread. General significance: Understanding these aspects identifies the N protein as a promising target for developing effective antiviral treatments and vaccines against SARS-CoV-2.

引用

页数：16

共 224 条

[1]

Abdul-Fattah S., 2021, Silico Modeling of Drugs Against Coronaviruses: Computational Tools and Protocols, P3

[2]

Al-Ejeh F., 2023, Omics Approaches and Technologies in COVID-19, P61

[3] Development of an IgG-Fc fusion COVID-19 subunit vaccine, AKS-452 [J].

Alleva, David G. ;

Delpero, Andrea R. ;

Scully, Melanie M. ;

Murikipudi, Sylaja ;

Ragupathy, Ramya ;

Greaves, Emma K. ;

Sathiyaseelan, Thillainaygam ;

Haworth, Jeffrey R. ;

Shah, Nishit J. ;

Rao, Vidhya ;

Nagre, Shashikant ;

Lancaster, Thomas M. ;

Webb, Sarah S. ;

Jasa, Allison, I ;

Ronca, Shannon E. ;

Green, Freedom M. ;

Elyard, Hanne Andersen ;

Yee, JoAnn ;

Klein, Jeffrey ;

Karnes, Larry ;

Sollie, Frans ;

Zion, Todd C. .

VACCINE, 2021, 39 (45) :6601-6613

[4] RNA interference, an emerging component of antiviral immunity in mammals [J].

Anobile, Dario Pasquale ;

Poirier, Enzo Z. .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2023, 51 (01) :137-146

[5]

[Anonymous], 2006, Safety and immunogenicity study of a dengue virus DNA vaccine

[6]

Ashmawy R., 2024, Are the Comorbidities Constitute an Intrinsic Factor for Long COVID?, DOI [10.20944/preprints202405.0099.v1, DOI 10.20944/PREPRINTS202405.0099.V1]

[7] Cellular host factors for SARS-CoV-2 infection [J].

Baggen, Jim ;

Vanstreels, Els ;

Jansen, Sander ;

Daelemans, Dirk .

NATURE MICROBIOLOGY, 2021, 6 (10) :1219-1232

[8] The SARS-CoV-2 Nucleocapsid Protein and Its Role in Viral Structure, Biological Functions, and a Potential Target for Drug or Vaccine Mitigation [J].

Bai, Zhihua ;

Cao, Ying ;

Liu, Wenjun ;

Li, Jing .

VIRUSES-BASEL, 2021, 13 (06)

[9] Organokines in COVID-19: A Systematic Review [J].

Barbalho, Sandra Maria ;

Minniti, Giulia ;

Miola, Vitor Fernando Bordin ;

Haber, Jesselina Francisco dos Santos ;

Bueno, Patricia Cincotto dos Santos ;

Haber, Luiza Santos de Argollo ;

Girio, Raul S. J. ;

Detregiachi, Claudia Rucco Penteado ;

Dall'Antonia, Camila Tiveron ;

Rodrigues, Victoria Dogani ;

Nicolau, Claudia C. T. ;

Catharin, Virginia Maria Cavallari Strozze ;

Araujo, Adriano Cressoni ;

Laurindo, Lucas Fornari .

CELLS, 2023, 12 (10)

[10] Genus-specific pattern of intrinsically disordered central regions in the nucleocapsid protein of coronaviruses [J].

Barik, Sailen .

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 2020, 18 :1884-1890

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