Gut microbiota in adults with cystic fibrosis: Implications for the severity of the CFTR gene mutation and nutritional status

被引:0
作者
Durda-Masny, Magdalena [1 ]
Gozdzik-Spychalska, Joanna
Moranska, Katarzyna
Pawlowska, Natalia [1 ]
Mazurkiewicz, Michal [2 ]
Skrzypczak, Iwona [2 ]
Cofta, Szczepan [2 ]
Szwed, Anita [1 ]
机构
[1] Adam Mickiewicz Univ, Inst Human Biol & Evolut, Fac Biol, Uniwersytetu Poznanskiego 6 St, PL-61614 Poznan, Poland
[2] Poznan Univ Med Sci, Dept Pulmonol Allergol & Resp Oncol, Poznan, Poland
关键词
Gut microbiota; Cystic fibrosis; mutations in the CFTR gene; BMI; 16S rRNA;
D O I
10.1016/j.jcf.2024.06.016
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Background: Microbial dysbiosis has been linked to cystic fibrosis (CF); however, the composition of gut microbiota in adult CF patients in relation to severity of CF transmembrane conductance regulator (CFTR) gene mutation and nutritional status have not yet been explored. Study aimed to assess the gut microbiota composition in adults with CF, and its relationship with the severity of CFTR mutations, and BMI. Methods: Gut microbiota of 41 adults with CF, and 26 non-CF controls were compared using whole 16S rRNA gene sequencing. Differences in the microbial community between groups of patients classified according to the severity of CFTR mutations, and BMI were assessed. The alpha diversity, beta diversity, and taxa abundance were identified to reflect gut microbiota composition. Results: Results showed a significant decrease in alpha diversity of bacterial communities in CF compared to non- CF group, but no significant difference between the CF groups distinguished by the severity of CFTR mutations. However, more severe mutations were associated with the higher relative abundance of Bacteroides and Streptococcus and the lower relative abundance of Faecalibacterium and Blautia. . Undernourished CF patients showed significantly lower alpha diversity compared to non-CF group and CF patients with BMI within the norm. Significant differences in the structure of the gut microbiota between CF and non-CF groups, as well as between BMI groups were also found. Conclusions: Our research indicates that CF is associated with alterations in gut microbiota in adults. Additionally, in adult CF patients, the composition of the gut microbiota is also related to BMI.
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页码:977 / 983
页数:7
相关论文
共 30 条
[1]   The future of cystic fibrosis care: a global perspective [J].
Bell, Scott C. ;
Mall, Marcus A. ;
Gutierrez, Hector ;
Macek, Milan ;
Madge, Susan ;
Davies, Jane C. ;
Burgel, Pierre-Regis ;
Tullis, Elizabeth ;
Castaos, Claudio ;
Castellani, Carlo ;
Byrnes, Catherine A. ;
Cathcart, Fiona ;
Chotirmall, Sanjay H. ;
Cosgriff, Rebecca ;
Eichler, Irmgard ;
Fajac, Isabelle ;
Goss, Christopher H. ;
Drevinek, Pavel ;
Farrell, Philip M. ;
Gravelle, Anna M. ;
Havermans, Trudy ;
Mayer-Hamblett, Nicole ;
Kashirskaya, Nataliya ;
Kerem, Eitan ;
Mathew, Joseph L. ;
McKone, Edward F. ;
Naehrlich, Lutz ;
Nasr, Samya Z. ;
Oates, Gabriela R. ;
O'Neill, Ciaran ;
Pypops, Ulrike ;
Raraigh, Karen S. ;
Rowe, Steven M. ;
Southern, Kevin W. ;
Sivam, Sheila ;
Stephenson, Anne L. ;
Zampoli, Marco ;
Ratjen, Felix .
LANCET RESPIRATORY MEDICINE, 2020, 8 (01) :65-124
[2]   The altered gut microbiota in adults with cystic fibrosis [J].
Burke, D. G. ;
Fouhy, F. ;
Harrison, M. J. ;
Rea, M. C. ;
Cotter, P. D. ;
O'Sullivan, O. ;
Stanton, C. ;
Hill, C. ;
Shanahan, F. ;
Plant, B. J. ;
Ross, R. P. .
BMC MICROBIOLOGY, 2017, 17 :1-11
[3]   A review of cystic fibrosis: Basic and clinical aspects [J].
Chen, Qionghua ;
Shen, Yuelin ;
Zheng, Jingyang .
ANIMAL MODELS AND EXPERIMENTAL MEDICINE, 2021, 4 (03) :220-232
[4]   Gut Microbiota in Children With Cystic Fibrosis: A Taxonomic and Functional Dysbiosis [J].
Coffey, Michael J. ;
Nielsen, Shaun ;
Wemheuer, Bernd ;
Kaakoush, Nadeem O. ;
Garg, Millie ;
Needham, Bronwen ;
Pickford, Russell ;
Jaffe, Adam ;
Thomas, Torsten ;
Ooi, Chee Y. .
SCIENTIFIC REPORTS, 2019, 9 (1)
[5]   Ribosomal Database Project: data and tools for high throughput rRNA analysis [J].
Cole, James R. ;
Wang, Qiong ;
Fish, Jordan A. ;
Chai, Benli ;
McGarrell, Donna M. ;
Sun, Yanni ;
Brown, C. Titus ;
Porras-Alfaro, Andrea ;
Kuske, Cheryl R. ;
Tiedje, James M. .
NUCLEIC ACIDS RESEARCH, 2014, 42 (D1) :D633-D642
[6]   Enteric circular muscle dysfunction in the cystic fibrosis mouse small intestine [J].
De Lisle, R. C. ;
Sewell, R. ;
Meldi, L. .
NEUROGASTROENTEROLOGY AND MOTILITY, 2010, 22 (03) :341-349+e87
[7]   The determinants of survival among adults with cystic fibrosis-a cohort study [J].
Durda-Masny, Magdalena ;
Gozdzik-Spychalska, Joanna ;
John, Aleksandra ;
Czainski, Wojciech ;
Strozewska, Weronika ;
Pawlowska, Natalia ;
Wlizto, Jolanta ;
Batura-Gabryel, Halina ;
Szwed, Anita .
JOURNAL OF PHYSIOLOGICAL ANTHROPOLOGY, 2021, 40 (01)
[8]   Gut Microbiota Changes during Dimethyl Fumarate Treatment in Patients with Multiple Sclerosis [J].
Ferri, Caterina ;
Castellazzi, Massimiliano ;
Merli, Nicola ;
Laudisi, Michele ;
Baldin, Elisa ;
Baldi, Eleonora ;
Mancabelli, Leonardo ;
Ventura, Marco ;
Pugliatti, Maura .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (03)
[9]   Rapid Detection of Emerging Pathogens and Loss of Microbial Diversity Associated with Severe Lung Disease in Cystic Fibrosis [J].
Flight, William G. ;
Smith, Ann ;
Paisey, Christopher ;
Marchesi, Julian R. ;
Bull, Matthew J. ;
Norville, Phillip J. ;
Mutton, Ken J. ;
Webb, A. Kevin ;
Bright-Thomas, Rowland J. ;
Jones, Andrew M. ;
Mahenthiralingam, Eshwar .
JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (07) :2022-2029
[10]  
Garg Millie, 2017, Curr Gastroenterol Rep, V19, P6, DOI [10.1007/s11894-017-0546-0, 10.1007/s11894-017-0546-0]