Establishment of 3D cell culture systems with decellularized lung-derived extracellular matrix hydrogel scaffold

被引:0
作者
Subasi Can, Secil [1 ]
Tuncer, Sema [1 ]
Akel Bilgic, Hayriye [2 ]
Imrak, Gizem [2 ]
Guenal, Guelcin [1 ,3 ]
Damadoglu, Ebru [4 ]
Aydin, Halil Murat [1 ]
Karaaslan, Cagatay [1 ,2 ]
机构
[1] Hacettepe Univ, Inst Sci, Bioengn Div, Ankara, Turkiye
[2] Hacettepe Univ, Fac Sci, Dept Biol, Mol Biol Sect, Ankara, Turkiye
[3] Akdeniz Univ, Dept Plast Surg, Antalya, Turkiye
[4] Hacettepe Univ, Sch Med, Dept Chest Dis, Div Allergy & Immunol, Ankara, Turkiye
关键词
Decellularization; lung; hydrogels; extracellular matrix; air-liquid interface; PERFUSION DECELLULARIZATION; TISSUE; BIOMATERIALS; CHALLENGES;
D O I
10.1080/09205063.2024.2392356
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Decellularized tissue hydrogels, especially that mimic the native tissue, have a high potential for tissue engineering, three-dimensional (3D) cell culture, bioprinting, and therapeutic agent encapsulation due to their excellent biocompatibility and ability to facilitate the growth of cells. It is important to note that the decellularization process significantly affects the structural integrity and properties of the extracellular matrix, which in turn shapes the characteristics of the resulting hydrogels at the macromolecular level. Therefore, our study aims to identify an effective chemical decellularization method for sheep lung tissue, using a mixing/agitation technique with a range of detergents, including commonly [Sodium dodecyl sulfate (SDS), Triton X-100, and 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate] (CHAPS), and rarely used (sodium cholate hydrate, NP-40, and 3-[N,N-Dimethyl(3-myristoylaminopropyl)ammonio]propanesulfonate) (ASB-14). After the effectiveness of the used detergents on decellularization was determined by histological and biochemical methods, lung derived decellularized extracellular matrix was converted into hydrogel. We investigated the interactions between lung cells and decellularized extracellular matrix using proliferation assay, scanning electron microscopy, and immunofluorescence microscopy methods on BEAS-2B cells in air-liquid interface. Notably, this study emphasizes the effectiveness of ASB-14 in the decellularization process, showcasing its crucial role in removing cellular components while preserving vital extracellular matrix biological macromolecules, including glycosaminoglycans, collagen, and elastin. The resulting hydrogels demonstrated favorable mechanical properties and are compatible with both cell-cell and cell-extracellular matrix interactions.
引用
收藏
页码:22 / 44
页数:23
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